... Protocols Gibson Cloning Gibson Assembly Cloning You may also...to include in the Gibson reaction are different than with normal PCR primers (Gibson et al. , 2010). Number...new window) . Gibson Assembly® is licensed to New England Biolabs by TelesisBio. Gibson DG, Young L, Chuang...nmeth.1318 (Link opens in a new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). ...OpenWetWare: Gibson Assembly (Link opens in a new window) OpenWetWare: Janet Matsen’s guide to Gibson Assembly...Get started with Gibson Assembly Cloning! Protocols...Molecular Biology Protocols Summary In 2009 Dr. Daniel Gibson and colleagues at the J. Craig Venter Institute...

...the proper personal protective equipment (PPE) for BSL-1 and BSL-2 labs.... Protocols Personal Protective...Protective Equipment (PPE) for BSL-1 and BSL-2 Labs Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs...

...Proteins Michael Davidson Fluorescent Protein Collection Michael Davidson Fluorescent Protein Collection...Davidson lab fluorescent protein fusion constructs and backbones. Plasmid...Subcellular localization Optogenetics The lab of Michael Davidson from Florida State University has contributed ...localization data found below. In addition, the Davidson lab has contributed the corresponding empty backbone...with your gene of interest. Please visit Michael Davidson's Molecular Expressions website for additional ...

...Nucleolus (fibrillar center) 133964 SON-mEGFP AICSDP-82 mEGFP SON Nuclear speckles 159744 DMD-mEGFP AICSDP...

...tests. All sample comparisons Do you really need to know every pairwise comparison within your population...we will dive into the nitty gritty details of comparison statistical tests – when to use them, what they...one-tailed test also works for exclusively less-than comparisons. Paired and unpaired t-tests Samples that come...family-wise error rate by only performing the comparisons you really care about. Family-wise error is the...probability of a false positive occurring when many comparisons are performed. For example, if your alpha is ...you have 5 samples and perform every pairwise comparison (10 in total), your likelihood of a false positive...higher than if you only look at 2 or 3 pairwise comparisons. Similar to family-wise error, is the false discovery...

...eukaryotic genomes.  Components of a transposon system Transposon: The transposon is the DNA sequence that moves...also called retrotransposons, and Class II TEs, also called DNA transposons. DNA transposons are commonly...discovered transposons due to changes in coloration of corn kernels. Aside from corn, transposons are in ...2010).  So why do transposons matter? The outcome really depends on where a transposon lands. For example...components of transposons, how they are classified, and how they are used in the lab. What is a transposon? DNA...Transposable elements (TEs): Retrotransposons  Class I TEs are also known as retrotransposons. They transpose by...which are required for LTR retrotransposon transposition. Non-LTR retrotransposons contain two open reading...

...do an AMA in the first place and give you some reasons why you should consider using Reddit to share your... as our first foray into Reddit. Here are some reasons why: 1. Reddit has a large and active community...

...research findings so difficult to do?  There are four reasons that research can be difficult to replicate. Unclear...with powerful cognitive biases such as motivated reasoning and hindsight bias, we may fool ourselves and ...Unanticipated discoveries Finally, there are good reasons for obtaining a different result after conducting...

...contact to connect on LinkedIn and always include a personal note about where you met and what you discussed...the near future. Even just one more meeting (in person or virtually) will make the relationship stronger... law of networking--don’t be creepy. There is a person on LinkedIn I once helped by answering one simple...not at all necessary. Once you’ve talked with a person a few times, if it was a positive experience for...was in graduate school, there was only one other person in my class who had children. When I had my baby...answered my questions and honestly, she was the only person who really understood what I was going through....

...conversations on the history of the group, lessons learned, and to Sonali Roy, Magdalena Julkowska, and Jennifer... provide greater visibility of these materials, Sonali Roy, a postdoctoral researcher at the Noble Research...effort. The future of reproducibility workshops and lessons learned: Since early 2018 the team has grown to...researchers at all levels of the scientific community. Lessons learned Instead of continuously talking about...

...the next steps. Harvard PI Angela DePace has put personal development at the center of her lab (pictured...easily adaptable for use in your lab! Promoting personal development through thoughtful planning and honest... of in the supplement of this paper), help each person organize his or her thoughts to facilitate honest...through obstacles, and take steps to resolve interpersonal conflicts in the lab. Yearly planning meetings...hear about the steps taken in your lab to promote personal development - let us know your strategies in the... personal development...

...Zhang group. Mechanism of CRISPR-transposons One of the CRISPR-transposon systems developed by the Sternberg...aka mini-transposon), which contains the DNA cargo of interest flanked by ~50-150 bp transposon end sequences...characteristic of the Tn7-family of transposons. Insertion of the mini-transposon can occur in two different orientations...with the same transposon exhibit reduced efficiencies (Vo et al., 2020). CRISPR-transposons within the CRISPR...Lab at Columbia University Medical Center. DNA transposons are ubiquitous genetic elements capable of spreading... Beauty). Despite their diverse utility, DNA transposons have been hindered by their limited programmability...recently surpassed with the discovery of CRISPR-transposons by the Sternberg and Zhang labs (Peters et al...

... said Addgene’s Lianna Swanson, who has been working with members of Davidson's lab to coordinate the ...work of Michael Davidson and his colleagues." Acknowledgments Thank you to Michael Davidson, Michelle Baird...and their use for imaging cell biology, Michael Davidson’s lab at Florida State University has been the ... easily searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection webpage. These...who has created plasmids as a technician in the Davidson lab for the last four years. “Researchers can ...comprehensive list of readily available targets since the Davidson lab always tagged new proteins with mEmerald first...to localize to specific cellular structures.The Davidson lab even included some of their own reference ...

...announce the recipients of this year’s Michael Davidson and Roger Tsien Commemorative Conference Awards...of their choice while honoring the late Michael Davidson and Roger Tsien who have played enormous roles...made some changes. In addition to supporting in-person conference attendance, the award now supports virtual... Aggarwal, and colleagues Filip Tomaska and Tim Hanson, are characterizing variants of iGluSnFR3 in vivo...

...the basics of Gibson assembly as a parody of “Breakfast at Tiffany’s.” Overview of Gibson assembly The ...The Gibson assembly technique was first described by Dr. Daniel Gibson and colleagues at the J. Craig Venter...program to design primers for Gibson Assembly. For a simple example of using Gibson assembly, imagine that you...the mixing and matching of parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated... a try! References: 1. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO. Enzymatic... have become more and more common, and for good reason. They offer many advantages over the traditional...disadvantages, and examples of how scientists are using Gibson assembly to put together DNA fragments. As a fun...

...Housden BE, Perrimon N, Collins JJ, Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods...976 . https://doi.org/10.1038/nmeth.2600 Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP...