We narrowed to 6,351 results for: Rel

1. CMV-CASANOVA

   Plasmid

   #113035

   Purpose

   CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

   Plasmid

   #126698

   Purpose

   to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

   Insert

   sgRNA targeting human SCGB3A2 locus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

   Plasmid

   #140197

   Purpose

   Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Insert

   dLbCas12a-VPR
   

   Use

   CRISPR and Synthetic Biology; Expression in asper…

   Tags

   VPR (VP64-p65-Rta), NLS

   Expression

   Mutation

   D832A DNase deactivated

   Promoter

   PgpdA

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1+ Flag-RAP2A

   Plasmid

   #128099

   Purpose

   Expresses RAP2A in mammalian cells in a G418-selectable plasmid

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2xmyc-RAB10

   Plasmid

   #164631

   Purpose

   Expresses RAB10 in mammalian cells with a 2xmyc tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   Ras-related protein Rab-10 (RAB10 Human)
   

   Use

   Tags

   2xmyc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFRT/TO/HIS/FLAG/HA-EDF1

   Plasmid

   #38079

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   EDF1 endothelial differentiation-related factor 1 (EDF1 Human)
   

   Use

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTriEX-Antenna-GDI Rac1

   Plasmid

   #83359

   Purpose

   Expression of Antenna-Rac1

   Depositor

   Klaus Hahn

   Article

   Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   FRET Antenna (mCerulean-cpVenus) and His

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2xmyc-RAB35

   Plasmid

   #164632

   Purpose

   Expresses RAB35 in mammalian cells with a 2xmyc tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   RAB35 (RAB35 Human)
   

   Use

   Tags

   2xmyc

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSGFPnERT(#205)

   Plasmid

   #184065

   Purpose

   cyclofen-inducible GFP nuclear relocation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Article

   Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d

   Insert

   eGFP-nls-ERT2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   sCMV+SP6

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1+ Flag-RAGB99L

    Plasmid

    #128101

    Purpose

    Expresses constitutively GTP-bound RAGB in mammalian cells in a G418-selectable plasmid

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    Ras related GTP binding B (RRAGB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q99L (CAA to CTA)

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-puro HA-Atg14*dCC

    Plasmid

    #38266

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    autophagy related genes 14 (ATG14 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    changed A618T, A621T, C624A and T627C for siRNA r…

    Promoter

    LTR

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pBabe-PI(WT)-Rac1(WT)-mCherry

    Plasmid

    #87550

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive Rac1

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Rac1 (RAC1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-PI(WT)-Rac1(Q61L)-mCherry

    Plasmid

    #87553

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Rac1 (RAC1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Q61L

    Promoter

    CMV

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-2T-TRR-C421-Y2404F

    Plasmid

    #182846

    Purpose

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    TRR 2011-2431 (trr Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. ArpC3-FLAG_pLib

    Plasmid

    #173680

    Purpose

    ArpC3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC3 (ARPC3 Human)
    

    Use

    Tags

    TEV cleavage site and FLAG tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. RCAS-sgATG7

    Plasmid

    #75228

    Purpose

    An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

    Depositor

    Noor Gammoh

    Article

    Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

    Insert

    Autophagy Related 7 (Atg7 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

    Plasmid

    #62577

    Purpose

    Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pEMS1210

    Plasmid

    #29107

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple238 (TRH Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT-hMAPT

    Plasmid

    #231583

    Purpose

    Minigene of human MAPT containing exon 9, 10, 11 and relevant intronic sequences

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Cell Genom. 2024 Jun 12;4(6):100563. doi

    Insert

    human MAPT (MAPT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. OPRL1-DuET

    Plasmid

    #213360

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPRL1 (OPRL1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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