We narrowed to 9,184 results for: Pol;

1. pEv-Fat4-citrine

   Plasmid

   #112854

   Purpose

   Constitutive expression of human fat4 protein fused to citrine fluorescent protein

   Depositor

   David Sprinzak

   Article

   Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

   Insert

   FAT4 (FAT4 Human)
   

   Tags

   Citrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. TDP-43_NTD1-80_E17R

   Plasmid

   #104479

   Purpose

   bacterial expression of TDP-43 NTD E17R

   Depositor

   Nicolas Fawzi

   Article

   Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   E17R

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEP-LAMP1-RFP

   Plasmid

   #167970

   Purpose

   Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

   Depositor

   Juan Bonifacino

   Article

   Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Insert

   LAMP1 (Lamp1 Rat)
   

   Tags

   SEP and mRFP

   Expression

   Mammalian

   Mutation

   D50E

   Promoter

   CMV

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_AR-RE_MLPmin_BC1011-luc2

   Plasmid

   #227106

   Purpose

   Androgen receptor response element for luciferase and barcode assays; barcode BC1011

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   8x clustered AR response element linked to MLP minimal promoter driving barcode 1011 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-Dlx-SynaptoTAG

   Plasmid

   #210729

   Purpose

   AAV vector for mapping synaptic projections. GABAergic neuron-specific Dlx promoter expresses EGFP-Synaptobrevin-2 to label synaptic terminals and Palm-tdTomato to label neuronal soma and axons.

   Depositor

   Wei Xu

   Article

   Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

   Insert

   EGFP-Synaptobrevin-2, P2A, Palm-tdTomato (tdTomato with Palmitoylation sequence) (Vamp2 Synthetic, Rat)
   

   Use

   AAV

   Promoter

   Dlx

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

   Plasmid

   #174515

   Purpose

   Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Inserts

   Mm PylS
   MmtRNA-Pyl-opt(UGA)
   

   Expression

   Bacterial

   Mutation

   ASL from the serT gene (UGA anticodon) and recoded

   Promoter

   GlnS and lpp

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM1 TMEM192-Flag-FKBP

   Plasmid

   #112743

   Purpose

   express TMEM192-Flag-FKBP

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   TMEM192-Flag-FKBP (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   Flag, FKBP

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRE-Lepr_FL-IRES-eGFP

   Plasmid

   #196635

   Purpose

   Doxycycline inducible expression of full length mouse Lepr with eGFP marker

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Insert

   Lepr (Lepr Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PDG459 V3

   Plasmid

   #226958

   Purpose

   CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. SaCas9 GFP V3

    Plasmid

    #226962

    Purpose

    CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. peGFP C3-SPG20 8xV

    Plasmid

    #218579

    Purpose

    Expresses GFP-tagged human SPG20 bulky hydrophobic amino acid mutant in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    SPG20 (SPART Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed Tryptophan 419, Leucine 438, Isoleucine 4…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Frt-hspB3

    Plasmid

    #63094

    Purpose

    Expression of HSPB3 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb3 (HSPB3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. SaCas9v3-Puro

    Plasmid

    #178813

    Purpose

    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIB-HIS-TEV-CKS1

    Plasmid

    #177013

    Purpose

    Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CKS1 (CKS1B Synthetic, Human)
    

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. EBFP2-UtrCH

    Plasmid

    #124656

    Purpose

    F-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    Utrophin (UTRN Human)
    

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    see depositor comments below

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. HaloTag-LaminA

    Plasmid

    #231894

    Purpose

    Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    LaminA (LMNA Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pN094

    Plasmid

    #167155

    Purpose

    MoClo-compatible Level1 (position 6) vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-npgA-ocsT
    

    Expression

    Plant

    Promoter

    p35s

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry-N1-EXO1b

    Plasmid

    #111621

    Purpose

    Expression of human WT Exonuclease 1 (EXO1) tagged with mCherry in mammalian cells

    Depositor

    Marco Muzi-Falconi

    Article

    Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

    Insert

    Exonuclease 1b (EXO1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET32a-HD46Q

    Plasmid

    #11515

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 46Q glutamines (HTT Human)
    

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 46 glutamines, vector m…

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZac2.1 GfaABC1D hM3D mCherry SV40

    Plasmid

    #92284

    Purpose

    AAV DREADD expression

    Depositor

    Baljit Khakh

    Article

    Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

    Insert

    hM3D (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Promoter

    GfaABC1D

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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