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Showing: 6221 - 6240 of 20007 results

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pCF821-sgCIDE-32_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211676

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Publication

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-32 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCF821-sgCIDE-34_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211678

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Publication

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-34 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCF821-sgRPA1-1_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211658

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Publication

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgRPA1-1 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCF821-sgCIDE-41_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211681

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Publication

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-41 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMLS640

Plasmid

#188892

Purpose

ttTi5605 MosSci targeting vector with Pmex-5::Cas9 and floxed Cbr-unc-119 marker

Depositor

Erik Jorgensen

Publication

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9, Cbr-unc-119

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS538

Plasmid

#188881

Purpose

Gateway Entry Vector [1-2]: 2xNLS::Cas9 (with 4 introns from smu-2)

Depositor

Erik Jorgensen

Publication

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Cas9

Use

Gateway Cloning

Mutation

C. elegans codom optimization and intron addition

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pODN-H2BC11-TagBFP

Plasmid

#183867

Purpose

Repair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 homology arms with linker-mTagBFP (H2BC11 Human)

Use

CRISPR; Donor template

Tags

linker-mTagBFP

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-ECT2_sgRNA

Plasmid

#183875

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ECT2 sgRNA spacer (ECT2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-CfANLN_sgRNA

Plasmid

#183879

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN sgRNA spacer

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-MYH10_sgRNA

Plasmid

#183887

Purpose

pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

MYH10 sgRNA spacer (MYH10 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-H2BC11_sgRNA

Plasmid

#183881

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 sgRNA spacer (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-CfANLN_sgRNA

Plasmid

#183880

Purpose

pX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN sgRNA spacer

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-MYH10_sgRNA

Plasmid

#183886

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

MYH10 sgRNA spacer (MYH10 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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Ash1

Plasmid

#169711

Purpose

Bacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)

Depositor

Tanja Mittag

Publication

Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

Insert

Ash1 (ASH1 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

Truncation including encoding amino acids 420-500

Promoter

T7

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD

Plasmid

#162811

Purpose

Control STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_(C1A)-Intein_DD

Use

Piggybac

Expression

Mammalian

Mutation

Intein(Cys1,Ala)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

Plasmid

#162805

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA_IRES_Blasticidin

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MD2 (F126A)-HA

Plasmid

#133067

Purpose

Retroviral expression of mouse MD2 (F126A) tagged with HA

Depositor

Jonathan Kagan

Publication

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (F126A) (Ly96 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

F126A

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MD2 (C95A)-HA

Plasmid

#133066

Purpose

Retroviral expression of mouse MD2 (C95A) tagged with HA

Depositor

Jonathan Kagan

Publication

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (C95A) (Ly96 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

C95A

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 NT sgRNA3

Plasmid

#164929

Purpose

Expresses Non-targeting sgRNA3 control in mammalian cells

Depositor

Ji Luo

Publication

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

Non-targeting sgRNA3 (verified for knockout)

Use

Lentiviral

Promoter

EFS promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-TAZ-S89A/S311A/S51A

Plasmid

#213590

Purpose

Doxycycline inducible expression of TAZ-S89A/S311A/S51A cDNA in mammalian cells

Depositor

Channing Der

Publication

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

2xHA

Expression

Mammalian

Mutation

S89A, S311A, S51A

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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