We narrowed to 9,184 results for: Pol;

1. pEGFP Cep170 (Nigg PID200)

   Plasmid

   #41150

   Depositor

   Erich Nigg

   Article

   Guarguaglini et al Mol Biol Cell. 2005 Mar;16(3):1095-107.

   Insert

   Cep170 (CEP170 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AN-DDK_WT RAD23A

   Plasmid

   #201438

   Purpose

   Expresses human RAD23A with an N-terminal FLAG tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. ABEmax-Puro V3

   Plasmid

   #226956

   Purpose

   ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI-MS2V5-eIF4G delN83

   Plasmid

   #65812

   Purpose

   expression of eIF4G-MS2 fusion.

   Depositor

   Niels Gehring

   Article

   Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

   Insert

   eIF4G (EIF4G1 Human)
   

   Tags

   MS2 stem loops and V5

   Expression

   Mammalian

   Mutation

   delN83 (functionally behaves as the WT protein)

   Promoter

   CMV

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

   Plasmid

   #74102

   Purpose

   plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Xenopus laevis beta-tubulin gene promoter region and 5'UTR
   Green Lantern Green Fluorescent Protein
   Rabbit beta 1-globin gene 3'UTR
   

   Expression

   Bacterial

   Available Since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-EF1A-DTX2-1-178-EGFP-Hygro

   Plasmid

   #238094

   Purpose

   Expression of structurally predicted WWE domain of DTX2 with EGFP on C terminal

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   DTX2 (DTX2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-178 only

   Promoter

   EF1alpha

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-CDH5-mRuby3-IRES-puro

   Plasmid

   #85144

   Purpose

   lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)
   

   Use

   Lentiviral

   Tags

   mRuby3

   Promoter

   CAG

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET32a-HD25Q

   Plasmid

   #11508

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 25 glutamines (HTT Human)
   

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 25 glutamines, vector m…

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_6xHis-PARP1-CAT

   Plasmid

   #173943

   Purpose

   Bacterial expression of isolated PARP1 CAT domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CAT (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)

    Plasmid

    #169186

    Purpose

    To express His-SUMO-nsp7 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
    

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-hCdt1-HA

    Plasmid

    #117500

    Purpose

    Expresses human Cdt1 fused to HA tag

    Depositor

    Domenico Maiorano

    Article

    Tsanov et al Nucleic Acids Res. 2014 Apr;42(6):3692-7

    Insert

    Cdc10-dependent transcript-1 (CDT1 1700 bp, Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available Since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmirGLO-3'UTR-TNF

    Plasmid

    #153069

    Purpose

    Human TNF 3'UTR region cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    3' UTR region of TNF gene (TNF Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_GR-RE_MLPmin_BC1092-luc2

    Plasmid

    #227112

    Purpose

    Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

    Plasmid

    #127177

    Purpose

    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Tags

    Flag- HA- Tandem Epitopes

    Promoter

    None

    Available Since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Pdg459-eSp(1.1) V3

    Plasmid

    #226965

    Purpose

    CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac1-His10FLAG

    Plasmid

    #157736

    Purpose

    empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

    Depositor

    Debra Hansen

    Article

    Olson et al Protein Expr Purif. 2021 May 7:105890. d

    Type

    Empty backbone

    Tags

    His10 + 3xFLAG, enterokinase-cleavable

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28_6xHis-PARP1-Zn2

    Plasmid

    #174805

    Purpose

    Bacterial expression of isolated PARP1 Zn2 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Zn2 (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28_6xHis-PARP1-Zn3

    Plasmid

    #174806

    Purpose

    Bacterial expression of isolated PARP1 Zn3 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Zn3 (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCWXPG-UBI-Ngn3PDX1MafA

    Plasmid

    #114303

    Purpose

    Constitutive expression of Ngn3, PDX1 and MafA proteins from a precursor with self-cleavable 2A peptides

    Depositor

    Patrick Salmon

    Article

    Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

    Insert

    Ngn3 (NEUROG3 Human)
    

    Use

    Lentiviral

    Mutation

    Ngn3, PDX1 and MafA proteins are expressed from a…

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Frt-V5-hspB8

    Plasmid

    #63109

    Purpose

    Expression of HSPB8 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb8 (HSPB8 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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