We narrowed to 13,804 results for: TIM

1. CD2 gRNA (BRDN0001146864)

   Plasmid

   #75827

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CD2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CD2 (CD2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGST-HsSyx-375-792

   Plasmid

   #179292

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Use

   Tags

   His6 + GST + TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   residues 375-792 from accession number NP_0010361…

   Promoter

   T7

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-PARP1_D770A-mCherry

   Plasmid

   #192260

   Purpose

   Expresses PARP1 D770A mutant in mammalian cells. Tagged with mCherry.

   Depositor

   Michael Cohen

   Article

   Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

   Insert

   PARP1_D770A (PARP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D770A

   Promoter

   CMV

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PARP1_R878A-mCherry

   Plasmid

   #192261

   Purpose

   Expresses PARP1 R878A mutant in mammalian cells. Tagged with mCherry.

   Depositor

   Michael Cohen

   Article

   Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

   Insert

   PARP1_R878A (PARP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R878A

   Promoter

   CMV

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-xBE3-P2A-Puro

   Plasmid

   #110870

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-ProDOL

   Plasmid

   #206866

   Purpose

   Expression of ProDOL probe - LynAnchor-eGFP-SNAP-tag-HaloTag

   Depositor

   Dirk-Peter Herten

   Article

   Tashev et al Nat Methods. 2024 Sep;21(9):1708-1715. d

   Insert

   ProDOL
   

   Use

   Retroviral

   Tags

   HaloTag, His-Tag, SNAP-tag, and eGFP

   Expression

   Mammalian

   Mutation

   Contains Lyn amino acids 1-16

   Promoter

   Available since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-SFXN2_STOP

   Plasmid

   #161422

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SFXN2 (SFXN2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. CSK gRNA (BRDN0001149071)

   Plasmid

   #77768

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CSK

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CSK (CSK Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

   Plasmid

   #114089

   Purpose

   CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized FnCas12a
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-syn-FLEX-jYCaMP1

    Plasmid

    #135422

    Purpose

    Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Insert

    jYCaMP1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221-SLC25A26_STOP

    Plasmid

    #161425

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A26 (SLC25A26 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET29-hMIGA2(170-593)-6xhis

    Plasmid

    #192834

    Purpose

    Express codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CDK14 gRNA (BRDN0001147002)

    Plasmid

    #76638

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK14

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK14 (CDK14 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CDK14 gRNA (BRDN0001146521)

    Plasmid

    #76639

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK14

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK14 (CDK14 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CDK14 gRNA (BRDN0001148240)

    Plasmid

    #76640

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK14

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK14 (CDK14 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CDK2 gRNA (BRDN0001148866)

    Plasmid

    #77191

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK2 (CDK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-EGFP.T2A.NCLX

    Plasmid

    #181873

    Purpose

    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Inserts

    solute carrier family 8 member B1 (Slc8a1 Mouse)
    EGFP
    

    Use

    AAV and Adenoviral

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    synthetic hybrid CAG promoter and synthetic hybri…

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMY-CD3-P2A-CD90.2

    Plasmid

    #163338

    Purpose

    Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the LTR promoter

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD3
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL

    Plasmid

    #119952

    Purpose

    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    gpps
    limS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal truncation of 56 amino acid residues (…

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. GSK3B gRNA (BRDN0001145264)

    Plasmid

    #77281

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GSK3B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    GSK3B (GSK3B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge