We narrowed to 9,173 results for: Pol

1. pET28_6xHis-PARP1-WGR

   Plasmid

   #174808

   Purpose

   Bacterial expression of isolated PARP1 WGR domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-WGR (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP457 pGK-dCas9-Suntag-BFP

   Plasmid

   #100957

   Purpose

   BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, SunTag array (10xGCN4)
   

   Use

   CRISPR

   Tags

   3xHA and BFP

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. mouse b8 full length

   Plasmid

   #217817

   Purpose

   Full length mouse integrin b8 expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGB8 (Itgb8 Mouse)
   

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET30a-His6-ABP-LOXCAT

   Plasmid

   #139971

   Purpose

   Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).

   Depositor

   Vamsi Mootha

   Article

   Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Insert

   A fusion of lactate oxidase from A. viridans and catalase from E. coli
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUb-Cas9-mCherry_cU6:6-sgRNA

   Plasmid

   #190598

   Purpose

   The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   mCherry - separated by T2A

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAP03-sp44/p21

   Plasmid

   #120232

   Purpose

   Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.

   Depositor

   Bradley Moore

   Article

   Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

   Insert

   bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
   

   Use

   Synthetic Biology

   Mutation

   aac(3)IV (apramycin resistance gene)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CRY-Gal∆DD (B1013)

   Plasmid

   #92035

   Purpose

   Expresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRES

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   Fusion of plant CRY2 to Gal4 binding domain (AA1-…

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CIB-VP64 (B1016)

   Plasmid

   #92037

   Purpose

   Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CIB1-VP64 (CIB1 Mustard Weed)
   

   Tags

   Fusion of plant CIB1 with VP64 activation domain.…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. CaV1.3e[8a,11,31b,Δ32,Δ42a]

   Plasmid

   #49332

   Purpose

   Repaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

   Depositor

   Diane Lipscombe

   Article

   Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

   Insert

   CACNA1D (Cacna1d Rat)
   

   Expression

   Mammalian

   Mutation

   Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

   Promoter

   CMV

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. AT-U1

    Plasmid

    #112059

    Purpose

    U1 snRNA tagged with 1 copy of Riboglow (truncated variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    U1 (RNU1-1 Human)
    

    Expression

    Mammalian

    Mutation

    1 copy of Riboglow tag (truncated variant A) near…

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe-Puro-IKBalpha-wt

    Plasmid

    #15290

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    inhibitor of Kappa B alpha (NFKBIA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

    Plasmid

    #164471

    Purpose

    Expresses sgRNA targeting human GJA1 locus

    Depositor

    Markus Kuehn

    Article

    Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

    Insert

    Gap junction alpha-1 (GJA1) (GJA1 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_AR-RE_MLPmin_BC1014-luc2

    Plasmid

    #227108

    Purpose

    Androgen receptor response element for luciferase and barcode assays; barcode BC1014

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHRIG-AktDN

    Plasmid

    #53597

    Purpose

    lentiviral expression of dominant negative mouse Akt1 and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Lentiviral

    Tags

    HA and IRES-EGFP

    Expression

    Mammalian

    Mutation

    Dominant negative (K179M)

    Promoter

    CMV

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pAAV_ER-RE_MLPmin_BC1063-luc2

    Plasmid

    #227109

    Purpose

    Estrogen receptor response element for luciferase and barcode assays; barcode BC1063

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBetaActin-FKBP-mScarlet-Dync1h1motor

    Plasmid

    #191330

    Purpose

    Expresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
    

    Expression

    Mammalian

    Mutation

    DYNC1H1 motor domain aa 1453-4644

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Zeo_BACE1

    Plasmid

    #227105

    Purpose

    BACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assays

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    BACE1 (BACE1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNK5712

    Plasmid

    #219752

    Purpose

    pGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pGAP - nnH3H_v2 - tAOX
    

    Expression

    Yeast

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX_BHLHE40-FlagHA_IRES-ZsGreen1

    Plasmid

    #149714

    Purpose

    Lentiviral vector expressing human BHLHE40 with FLAG-HA tags

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    BHLHE40 (BHLHE40 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBK086

    Plasmid

    #183145

    Purpose

    pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

    Depositor

    Daan Swarts

    Article

    Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

    Insert

    MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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