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pME_G_0_1-2_001_OC5'1

Plasmid

#235801

Purpose

5' Operon Connector 1 in the M1 position used to construct operons

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 1

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1_008_5C2SF

Plasmid

#235790

Purpose

5' Connector 2 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 2 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1_011_5C3SF

Plasmid

#235791

Purpose

5' Connector 3 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 3 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1_022_5C4SF

Plasmid

#235792

Purpose

5' Connector 4 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 4 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1_014_5C5SF

Plasmid

#235793

Purpose

5' Connector 5 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 5 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_G_0_1_005_5C1SF

Plasmid

#235789

Purpose

5' Connector 1 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 1 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_Cp_0_7-8_004_Amp/ColEI_sfGFP

Plasmid

#235970

Purpose

Ampicillin/ColEI backbone with sfGFP placeholder for lvl1 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Amp/ColEI

Use

Synthetic Biology

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLX304 mNG PH PLCD1

Plasmid

#233254

Purpose

Expression of mNeonGreen-tagged PH domain of PLCD1

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

PLCD1

Use

Lentiviral

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6-CFAPoptim-NAT

Plasmid

#221129

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::NATMX

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim

Expression

Yeast

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-SEC61-FAPoptim

Plasmid

#221126

Purpose

FAP-tagged marker for the ER (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC61-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-STE3-GFP

Plasmid

#221113

Purpose

GFP-tagged STE3 (C-terminally tagged, optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

STE3-GFP

Expression

Yeast

Promoter

TEF1

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7_Sr62NLRa

Plasmid

#233528

Purpose

Expresses Sr62NLRa in yeast

Depositor

Brande Wulff

Article

Chen et al bioRxiv 2024.08.30.610287

Insert

Sr62NLRa

Tags

GAL4 AD, HA tag

Expression

Yeast

Promoter

ADH1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-CUP1pr-NFAPoptim

Plasmid

#221110

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-CUP1pr-CFAPoptim

Plasmid

#221111

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-TEF1pr-RPA34-FAPoptim

Plasmid

#221123

Purpose

FAP-tagged marker for the nucleus (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

RPA34-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-TEF1pr-NFAPoptim

Plasmid

#221088

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-TEF1pr-CFAPoptim

Plasmid

#221089

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-TEF1pr-NFAPoptim

Plasmid

#221093

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-TEF1pr-CFAPoptim

Plasmid

#221094

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-ADH1pr-NFAPoptim

Plasmid

#221096

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge