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pCMV 3xFLAG-GABARAP G116

Plasmid

#123099

Purpose

Expresses 3xFLAG-GABARAP G116 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-LC3B2-GFP

Plasmid

#123109

Purpose

Expresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL1 G116A

Plasmid

#123101

Purpose

Expresses 3xFLAG-GABARAPL1 G116A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-DU422gp140-TM

Plasmid

#123251

Purpose

Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tether

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (DU422) Env

Tags

CD5 leader peptide and Linker (with 6his tag) and…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pZC13 (pFA6a-TEV-6xGly-V5-HphMX)

Plasmid

#44085

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

6xGly linker, ADH1 transcription terminator, HphM…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

Plasmid

#223159

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-ANO6/TMEM16F [N429/19]

Plasmid

#190327

Purpose

Mammalian Expression Plasmid of anti-ANO6/TMEM16F (Human). Derived from hybridoma N429/19.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-ANO6/TMEM16F (Homo sapiens) recombinant Mouse monoclonal antibody (ANO6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TARPGamma2/Stargazin [N245/1R]

Plasmid

#177521

Purpose

Mammalian Expression Plasmid of anti-TARPGamma2/Stargazin (Rat). Derived from hybridoma N245/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TARPGamma2/Stargazin (Rattus norvegicus) recombinant mouse monoclonal antibody (Cacng2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Dopamine D2 receptor [N186/29R]

Plasmid

#177509

Purpose

Mammalian Expression Plasmid of anti-Dopamine D2 receptor (Rat). Derived from hybridoma N186/29.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Dopamine D2 receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Drd2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-CASPR2 [K67/25R]

Plasmid

#177446

Purpose

Mammalian Expression Plasmid of anti-CASPR2 (Human). Derived from hybridoma K67/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-CASPR2 (Homo sapiens) recombinant mouse monoclonal antibody (CNTNAP2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.blast-Flag-MFF-S155,172A

Plasmid

#74380

Purpose

Lentiviral expression vector containing flag tagged S155,172A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Ser 155 Ala, Ser 172 Ala (Numbering based on MFF …

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only
pMXs-ms-L-Myc-L351P

Plasmid

#50777

Purpose

retroviral expression of mouse L-Myc L351P mutant

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

L-Myc-L351P (Mycl Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

L351P (does not bind to Max)

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-hPCM2w/b delta6

Plasmid

#47588

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2w/b delta6 (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

6 C-terminal AA deleted (needed for PDZ binding)

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pTF279 (pFA6a-TEV-6xGly-2xStrep-HphMX)

Plasmid

#44082

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

2xStrep tag, 6xGly linker, ADH1 transcription ter…

Available Since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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pTF273 (pFA6a-PSc-6xGly-12xHis-HphMX)

Plasmid

#44075

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

12xHis tag, 6xGly linker, ADH1 transcription term…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pTF269 (pFA6a-PSc-6xGly-12xHis-KanMX)

Plasmid

#44087

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

12xHis tag, 6xGly linker, ADH1 transcription term…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTF278 (pFA6a-PSc-6xGly-2xStrep-KanMX)

Plasmid

#44088

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

2xStrep tag, 6xGly linker, ADH1 transcription ter…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-EPLIN alpha delta LIM

Plasmid

#40933

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Mutation

LIM domain deleted- amino acids 221-295

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp2-a del.SHD

Plasmid

#40050

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a del.SHD (SYTL2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of SHD domain (1-56)

Promoter

CMV promoter

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp2-a C2AB

Plasmid

#40051

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a C2AB (SYTL2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2AB domains only (aa 597-910)

Promoter

CMV promoter

Available Since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only