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SPDK3876(TRV-RNA2-pPEBV-MCS)

Plasmid

#149275

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAs

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter

Use

T-dna vector

Tags

Expression

Mutation

T2318C (DNA) in PEBV coat protein sequence

Promoter

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pICE-HA-MRE11-WT

Plasmid

#82033

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Patrick Calsou, Sébastien Britton

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

MRE11 (MRE11 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV-tet

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pTet-IRES-EGFP

Plasmid

#64238

Purpose

Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

Depositor

Maria Lung

Article

Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

pTet

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pFX-Tom20-ｍCherry

Plasmid

#174188

Purpose

Express mCherry with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with mitchondria-targeting sequence

Use

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDD427

Plasmid

#202080

Purpose

Frame +2/-1 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +2/-1 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mVenus

Plasmid

#198192

Purpose

Spectral correction: Expression of mVenus

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mVenus

Use

Tags

Expression

Mammalian

Mutation

A206K

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Puro.Cre empty vector

Plasmid

#17408

Depositor

Tyler Jacks

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2008

Availability

Academic Institutions and Nonprofits only

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Luciferase-pcDNA3

Plasmid

#18964

Depositor

William Kaelin

Article

Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

Insert

Firefly Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pC014 - LwCas13a-msfGFP

Plasmid

#91902

Purpose

Expresses active LwCas13a for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR

Tags

NLS and msfGFP-NLS-3xHA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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PB CAG-mTagBFP2-T2A-iCreERT2

Plasmid

#177308

Purpose

CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon

Depositor

Mario Capecchi

Insert

mTagBFP2-T2A-iCreERT2

Use

Cre/Lox; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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