163,606 results

1. MBP-CRY1-PHR

   Plasmid

   #26035

   Depositor

   Johann Deisenhofer

   Article

   Brautigam et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

   Insert

   CRY1 (CRY1 Mustard Weed)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pBabe puro-eIF4E

   Plasmid

   #33252

   Depositor

   Thomas Roberts

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   eIF4E (EIF4E Human)
   

   Use

   Retroviral

   Tags

   untagged

   Expression

   Mammalian

   Mutation

   unmodified

   Promoter

   SV40

   Available Since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

3. KE-009

   Plasmid

   #133441

   Purpose

   Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.

   Depositor

   Gerhard Wagner

   Insert

   EIF4H (EIF4H Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX305_FKBP12F36V-SHOC2

   Plasmid

   #134522

   Purpose

   Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag

   Depositor

   Andrew Aguirre

   Article

   Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

   Insert

   SHOC2 (SHOC2 Human)
   

   Use

   Lentiviral

   Tags

   FKBP12F36V-2xHA

   Expression

   Mammalian

   Mutation

   Silent mutations made at wobble base position at …

   Promoter

   hPGK

   Available Since

   Nov. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRST7-ER beta (short)

   Plasmid

   #11357

   Depositor

   Donald McDonnell

   Article

   Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

   Insert

   ER beta short (ESR2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 10, 2006

   Availability

   Academic Institutions and Nonprofits only

6. HC078 (3xHA-Atg13)

   Plasmid

   #59544

   Purpose

   Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

   Insert

   ATG13 (ATG13 Budding Yeast)
   

   Use

   Tags

   3xHA

   Expression

   Bacterial and Yeast

   Mutation

   AvrII site created after start codon

   Promoter

   ATG13

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pRK5M-TIMP3

   Plasmid

   #31715

   Depositor

   Rik Derynck

   Article

   Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

   Insert

   TIMP3 (TIMP3 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cas9-GFP_sg_mAMPKa2

   Plasmid

   #79005

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Prkaa2 (Prkaa2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

9. PP7 guide

   Plasmid

   #86168

   Purpose

   Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   PP7 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126706

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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