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pDONR223-MERTK

Plasmid

#23900

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MERTK (MERTK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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RB1-TP53-LRG-GFP

Plasmid

#225876

Purpose

Guides targeting TP53 and RB1

Depositor

Janai Carr-Ascher

Article

Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

Insert

RB1 and TP53 (TP53, RB1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-LYN

Plasmid

#23905

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LYN (LYN Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

Plasmid

#103871

Purpose

dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Inserts

PspCas13b
huADAR2DD (ADARB1 Human)

Use

CRISPR

Tags

GS and HIV NES

Expression

Mammalian

Mutation

Changed histidne 133 to alanine and histidine 105…

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-ACVR2A

Plasmid

#23654

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ACVR2A (ACVR2A Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-IRAK2

Plasmid

#23412

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

IRAK2 (IRAK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
MMK

Plasmid

#64121

Purpose

a modified episomal (EBNA1/OriP) vector expressing human Myc and KLF4 genes

Depositor

Linzhao Cheng

Article

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Insert

MYC-2A-KLF4 (MYC, KLF4 Human)

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN K577M

Plasmid

#125790

Purpose

constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN K577M (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

K577M (helicase-dead)

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN E84A

Plasmid

#125789

Purpose

constitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN E84A (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

E84A (exonuclease-dead)

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN E84A K577M

Plasmid

#125791

Purpose

constitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN E84A/K577M (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

E84A/K577M (exonuclease/helicase-dead)

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-DYRK1B

Plasmid

#23761

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DYRK1B (DYRK1B Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-DYRK4

Plasmid

#23825

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DYRK4 (DYRK4 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-ADPGK

Plasmid

#23809

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ADPGK (ADPGK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
MSCV-MYC-t2A-BCL2

Plasmid

#135306

Purpose

Overexpression of multiple human genes (BCL2 and MYC)

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

MYC (MYC Human)
BCL2 (BCL2 Human)

Use

Retroviral

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_BCR_ABL1

Plasmid

#205780

Purpose

Express mEGFP-tagged fusion protein, BCR_ABL1_p210 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

BCR_ABL1 (BCR, ABL1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_TMPRSS2_ERG

Plasmid

#205943

Purpose

Express mEGFP-tagged fusion protein, TMPRSS2_ERG from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TMPRSS2_ERG (TMPRSS2, ERG Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-FLT3

Plasmid

#23895

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLT3 (FLT3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-ERBB4

Plasmid

#23875

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ERBB4 (ERBB4 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PDGFRA

Plasmid

#23892

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRA (PDGFRA Human)

Use

Gateway donor vector

Mutation

M260I

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-LCK

Plasmid

#23890

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LCK (LCK Human)

Use

Gateway donor vector

Mutation

V93I

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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