We narrowed to 13,058 results for: BASE
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Plasmid#120089PurposeCyanobacteria synthetic plasmid harboring the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS) to test the pSYSA-TA1 toxin–antitoxin cassette.DepositorInsertpAM4787 with pSYSA-TA1 cassette cloned into the SacI restriction site.
UseOtherAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-AD8-QES.i08.c04
Plasmid#123259PurposeMammalian expression plasmid for Env from the AD8 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopesDepositorInsertHIV-1 (AD8) Env
TagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Mutations P124D, …PromoterCMVAvailable SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS116 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-Alfa-P2A-TagBFP
Plasmid#199369PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-SUMOstar-ALFA-P2A-TagBFPExpressionMammalianMutationWTAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-L2T1L
Plasmid#33287DepositorAvailable SinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNH11 (pmyo-2::Arch(D95N)::2xMycTag)
Plasmid#130275PurposeExpresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.DepositorInsertArch(D95N)
Tags2x Myc-tagExpressionWormAvailable SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-PXN
Plasmid#227316PurposeDonor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)DepositorInsertPXN Homology Arms flanking a mStayGold Tag (PXN Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mScarlet-H2BC11
Plasmid#207756PurposeDonor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
ABI_cPPVp
Plasmid#119212PurposeExpresses C-terminus of plum pox virus protease (PPVp) fused to the ABI-abscisic acid responsive protein domainDepositorInsertsplit cPPVp in fusion with ABI
TagsmycExpressionMammalianPromoterCMVAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
PYL1_nPPVp
Plasmid#119211PurposeExpresses N-terminus of plum pox virus protease (PPVp) fused to PYL1-abscisic acid responsive protein domainDepositorInsertsplit nPPVp in fusion with PYL1
TagsAU1 tagExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe)
Plasmid#105201PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe)DepositorInsertG8p-IL12-P2A-CCL21-1Pe
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceMarch 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRB_nSbMVp
Plasmid#118969PurposeExpresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domainDepositorInsertsplit nSbMVp in fusion with FRB
TagsMyc tagExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
TC1078-30
Plasmid#164878Purpose30bp(mismatch17) cas13d gRNA for 1405 editingDepositorInsertgRNA for TC1405
ExpressionMammalianAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-3HA
Plasmid#39283DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…Mutation-1163 to +6 of nmt1 locusAvailable SinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pASPIre1
Plasmid#154842PurposeDerivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sitesDepositorInsertBxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
ExpressionBacterialPromoterPrhaAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B3
Plasmid#172844PurposeCRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mTurquoise2
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GFP10-RHOB
Plasmid#182237PurposeExpresses RHOB GTPase with tripartite split GFP10 tagDepositorInsertras homolog family member B (RHOB Human)
TagsGFP10 (split-GFP tripartite)ExpressionMammalianPromoterCMVAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
B270 + SPRTN sgSTOP
Plasmid#100718PurposeB270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOPDepositorInsertsgSTOP targeting SPRTN (cloned using BbsI)
UseCRISPRExpressionMammalianPromoterU6 promotersAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-L2T3D
Plasmid#33277DepositorAvailable SinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
FKBP_cSbMVp
Plasmid#118968PurposeExpresses C-terminus of soybean mosaic virus protease (SbMVp) fused to the FK-506 binding protein (FKBP)DepositorInsertsplit cSbMVp in fusion with FKBP
TagsHA tagExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
LLP784_SnoopCatcher-DNMT3A-CO
Plasmid#211777PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertSpy-EZH2
Tags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only