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pcDNA-Evf2

Plasmid

#99478

Purpose

Mammalian expression of the Evf2 long non-coding RNA

Depositor

Jhumku Kohtz

Publication

Feng et al Genes Dev. 2006 Jun 1;20(11):1470-84. Ep

Insert

Evf2

Expression

Mammalian

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-6823

Plasmid

#84986

Purpose

For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Publication

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-PMKco-Mkco

Expression

Bacterial

Promoter

LacUV5

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K9R

Plasmid

#84527

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

none

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pDONR221-RAP1

Plasmid

#84525

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

no stop codon

Promoter

none

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSAH0016

Plasmid

#79824

Purpose

Dual selection plasmid with lac operator, selection for binding with spectinomycin, for non-binding with chloramphenicol

Depositor

Katja Arndt

Publication

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Inserts

cat
aadA

Promoter

AATTCAAAAAAATGCTTGACAATTAATCATCGGCTCGTATAATGTGTGG…

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-mOC-STAMP-GFP

Plasmid

#73579

Purpose

Lentivirus expression of mOC-STAMP

Depositor

Paul Odgren

Publication

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Insert

OC-STAMP (Ocstamp Mouse)

Use

Lentiviral

Tags

GFP

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
Pr2-T7RNAP

Plasmid

#67739

Purpose

OR2-OR1-P2 promoter expressing T7 RNAP

Depositor

Richard Murray

Publication

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-T7RNAP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr2

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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Pr2-deGFP-MGapt

Plasmid

#67736

Purpose

OR2-OR1-Pr2 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Publication

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr2

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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Pr1-deGFP-MGapt

Plasmid

#67735

Purpose

OR2-OR1-Pr1 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Publication

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr1

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-RACK1-NU

Plasmid

#255448

Purpose

Mammalian expression of HA-tagged human RACK1

Depositor

Christine Mayr

Publication

Luo et al Cell. 2026 Jun 8:S0092-8674(26)00576-3.

Insert

RACK1 (RACK1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-ENO1-NU

Plasmid

#255450

Purpose

Mammalian expression of HA-tagged human ENO1

Depositor

Christine Mayr

Publication

Luo et al Cell. 2026 Jun 8:S0092-8674(26)00576-3.

Insert

ENO1 (ENO1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pDASH-DIS-α2 (MoClo)

Plasmid

#242524

Purpose

Donor I vector in DASH and alpha2 vector in GoldenBraid/DASH, with SacB counter-selection, compatible with MoClo, with attBTT and attBCC-FRT

Depositor

Jose Alonso, Anna Stepanova

Publication

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-TurboID

Plasmid

#231293

Purpose

For transiently expressing fusion proteins with N terminal TurboID for proximity labeling in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

TurboID

Expression

Plant

Mutation

N/A

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-mEGFP

Plasmid

#231287

Purpose

For transiently expressing fusion proteins with N terminally tagged mEGFP in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mEGFP

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-mNeonGreen

Plasmid

#231288

Purpose

For transiently expressing fusion proteins with N terminally tagged mNeonGreen in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mNeonGreen

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-mCitrine

Plasmid

#231289

Purpose

For transiently expressing fusion proteins with N terminally tagged mCitrine in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mCitrine

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-mScarletI

Plasmid

#231290

Purpose

For transiently expressing fusion proteins with N terminally tagged mScarletI in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mScarletI

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-mCitrine

Plasmid

#231297

Purpose

For transiently expressing fusion proteins with C terminally tagged mCitrine in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mCitrine

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-mScarletI

Plasmid

#231298

Purpose

For transiently expressing fusion proteins with C terminally tagged mScarletI in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mScarletI

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-StrepIII

Plasmid

#231300

Purpose

For transiently expressing fusion proteins with C terminal StrepIII tags in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

StrepIII

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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