Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 17,659 results for: URE

Showing: 9421 - 9450 of 17659 results

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Efg1-ΔDBD-A51P/A56P/Q443P

Plasmid

#216402

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-A51P/A56P/Q443P

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56 and Glutamine 443 to Proli…

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y4S/Y8S/Y16S

Plasmid

#216405

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y4S/Y8S/Y16S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 4, 8 and 16 to Serine, and delet…

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-ΔDBD-Y64S/Y66S/Y69S

Plasmid

#216406

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y64S/Y66S/Y69S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 64, 66 and 69 to Serine, and del…

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-ΔDBD-Y101S/Y104S/Y106S

Plasmid

#216407

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y101S/Y104S/Y106S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 101, 104 and 106 to Serine, and …

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-ΔDBD-Y401S/Y404S/Y409S

Plasmid

#216409

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y401S/Y404S/Y409S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 401, 404 and 409 to Serine, and …

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-ΔDBD-Y450S/Y452S/Y456S

Plasmid

#216410

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y450S/Y452S/Y456S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 450, 452 and 456 to Serine, and …

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-ΔDBD-Y478S/Y482S/Y484S

Plasmid

#216411

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y478S/Y482S/Y484S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 478, 482 and 484 to Serine, and …

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GfaABC1D-Crym-BioID2-BioID2

Plasmid

#200070

Purpose

To study u-crystallin protein interactors

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

GfaABC1D-Crym-BioID2-BioID2

Use

AAV and Mouse Targeting

Tags

HA-tag

Promoter

GfaABC1D

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GfaABC1D-Crym-eGFP

Plasmid

#200080

Purpose

To study u-crystallin protein interactors, control plasmid

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

GfaABC1D-Crym-eGFP

Use

AAV and Mouse Targeting

Tags

eGFP

Promoter

GfaABC1D

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_human-pro-IL-18_E192K_D193K

Plasmid

#214322

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

E192K_D193K

Promoter

T7

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
rSirt3 shRNA (pLKO.1 vector)

Plasmid

#216807

Purpose

shRNA against rat Sirt3

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

Insert

Sirt3 (Sirt3 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_human-pro-IL-18_I48N

Plasmid

#214323

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

I48N

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pColdI-GST-mGTSF2

Plasmid

#215714

Purpose

Bacterial expression of GST-tagged GTSF2

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

GTSF2 (Gtsf2 Mouse)

Tags

6x His and GST

Expression

Bacterial

Promoter

CspA

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_mouse-Pro-IL-18_humanized-triple-mutant

Plasmid

#214339

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (Il18 Mouse)

Tags

His-TEV

Expression

Bacterial

Mutation

ENGD29DDEN_N36Y_H41E

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_human-pro-IL-18_LES33AAA

Plasmid

#214326

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

LES33AAA

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_human-pro-IL-18_DDEN29ENGD

Plasmid

#214325

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

DDEN29ENGD

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_human-pro-IL-18_V47N_I48N

Plasmid

#214324

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

V47N_I48N

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_rat

Plasmid

#214333

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (Il18 Rat)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-10_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211670

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-10 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-5_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211665

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-5 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-6_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211666

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-6 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-7_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211667

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-7 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgRPA1-2_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211659

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgRPA1-2 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-4_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211664

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-4 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-21_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211671

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-21 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-22_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211672

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-22 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgOR2B6-1_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211655

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgOR2B6-1 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgOR2B6-2_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211656

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgOR2B6-2 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgRPA1-3_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211660

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgRPA1-3 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-2_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211662

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-2 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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