We narrowed to 16,355 results for: Jun

1. pAcCas12nHS_RV6_empty

   Plasmid

   #203811

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEB1deltaC-mCherry

   Plasmid

   #80603

   Purpose

   Expresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus end

   Depositor

   Torsten Wittmann

   Article

   Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Truncated C-terminus; EB1 coding sequence ends at…

   Promoter

   CMV

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pN006

   Plasmid

   #188470

   Purpose

   Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   AsCas12a
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-Pur-CAG-hM3Dq-mCherry

   Plasmid

   #80948

   Purpose

   human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   hM3Dq-mCherry
   

   Use

   Tags

   mcherry fusion

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Bmpr2Δ751-813aa

   Plasmid

   #163603

   Purpose

   Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The LIMK-binding region (751-813 aa) is deleted

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pN256

   Plasmid

   #188472

   Purpose

   scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   eGFP-WPRE-DAISY-4T
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. V5-LOV-Turbo1-NIK3x_pTRE-G418

   Plasmid

   #202017

   Purpose

   expresses LOV-Turbo1-NIK3x in the mammalian nucleolus under dox inducible promoter, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   V5-LOV-Turbo1-NIK3x
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-Tight

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRIPZ shIFNAR

   Plasmid

   #127699

   Purpose

   Doxycyclin inducible shRNA knockdown of mouse IFNAR gene

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shIFNAR1 Mmu (Ifnar1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-ATXN1-30Q

   Plasmid

   #33238

   Depositor

   Huda Zoghbi

   Article

   Cummings et al Nat Genet. 1998 Jun;19(2):148-54.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 32 amino acids (30 Q…

   Promoter

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3 Flag MKK7b1(glu)

    Plasmid

    #14673

    Depositor

    Roger Davis

    Article

    Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

    Insert

    MKK7b1(Glu) (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-269Glu Thr-273Glu

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

11. AAVS1-Pur-CAG-hM4Di-mCherry

    Plasmid

    #80947

    Purpose

    human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

    Insert

    hM4Di-mCherry
    

    Use

    Tags

    mcherry fusion

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_FLAG-G1 PylRS

    Plasmid

    #154763

    Purpose

    plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. mcherry-Myo 9b R1695M

    Plasmid

    #134958

    Purpose

    Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.

    Depositor

    Martin Bähler

    Article

    Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

    Insert

    Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJY-RpABE-PDS3_IMS

    Plasmid

    #112875

    Purpose

    binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

    Depositor

    Jae-Young Yun, Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

    Inserts

    RPS5A promoter
    ABE7.10
    U6-sgRNA targeting PDS3
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5-myc-Miro1 K427V

    Plasmid

    #47892

    Purpose

    Expresses myc tagged Miro1 K427 constitutively active mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro1 K427V (RHOT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K427V, constitutively active mutant

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pDONR223-MCU

    Plasmid

    #31726

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19. ():.

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Expression

    Mutation

    *

    Promoter

    None

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Bmpr2ΔKinase

    Plasmid

    #163404

    Purpose

    Expresses BMPR-2 lacking the kinase domain in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The kinase domain (202-500 aa) is deleted

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. shCDK4/6(1)

    Plasmid

    #73554

    Purpose

    vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4 + shRNA against CDK6
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pBM019

    Plasmid

    #128179

    Purpose

    Construct for generating stable Cas9-expressing Toxoplasma gondii

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

    Inserts

    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3X FLAG and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-EBFP2-P2A-EGFP

    Plasmid

    #184938

    Purpose

    Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    EBFP2-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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