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BB1_L_12_syn_BsaI

Plasmid

#89914

Purpose

Linker to construct BB1 from PCR product with FS1 and FS2 (in with BsaI, out with BbsI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB1_L_34_syn_BsaI

Plasmid

#89916

Purpose

Linker to construct BB1 from PCR product with FS3 and FS4 (in with BsaI, out with BbsI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_AB_syn_BbsI

Plasmid

#89917

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSA and FSB (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_BC_syn_BbsI

Plasmid

#89918

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSB and FSC (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_CD_syn_BbsI

Plasmid

#89919

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSC and FSD (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_DE_syn_BbsI

Plasmid

#89920

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSD and FSE (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_FG_syn_BbsI

Plasmid

#89922

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSF and FSG (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_GH_syn_BbsI

Plasmid

#89923

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSG and FSH (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pQdCas12a.empty

Plasmid

#236036

Purpose

The plasmid pQdCas12a.empty expresses the dCas12a endonuclease.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Type

Empty backbone

Use

CRISPR

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHM028

Plasmid

#225522

Purpose

Encodes constitutive expression of mCherry

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Insert

mCherry

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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POC1400

Plasmid

#213121

Purpose

Testing the activity of BpiI-associated switch methylases

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Type

Empty backbone

Expression

Bacterial

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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POC1401

Plasmid

#213122

Purpose

Testing the activity of LguI-associated switch methylases

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Type

Empty backbone

Expression

Bacterial

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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POC1399

Plasmid

#213120

Purpose

Testing the activity of BsaI-associated switch methylases

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Type

Empty backbone

Expression

Bacterial

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGT270

Plasmid

#171878

Purpose

Start-Stop Level 2 destination vector for Synechocystis sp. PCC 6803

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Type

Empty backbone

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSM034

Plasmid

#128292

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - lp-sopB

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSPB847

Plasmid

#128297

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - parB

Expression

Bacterial

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pSPB848

Plasmid

#128299

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - lp-parB

Expression

Bacterial

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSM105

Plasmid

#128295

Purpose

asymmetric plasmid partitioning E. coli - negative control

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - lp-cfp

Expression

Bacterial

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSplit 1

Plasmid

#240576

Purpose

Destination vector for pSplit system in plants

Depositor

Xiaohan Yang

Article

Yuan et al Bio Protoc. 2025 Feb 20;15(4):e5214. doi

Insert

NpuDnaE(N)

Expression

Plant

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pUdO3t

Plasmid

#210248

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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