We narrowed to 9,173 results for: Pol

1. pGEX-6P-1_WT RAD23A

   Plasmid

   #201456

   Purpose

   Expresses human RAD23A with an N-terminal GST tag in E. coli

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   tac promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPICZ-mouseGIRK2-eGFP-StII-6xHis

   Plasmid

   #124277

   Purpose

   Expresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

   Depositor

   Arthur Laganowsky

   Article

   Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

   Insert

   GIRK2 (Kcnj6 Mouse)
   

   Tags

   eGFP-StreptagII-6xHis

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Frt-hspB8

   Plasmid

   #63099

   Purpose

   Expression of HSPB8 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb8 (HSPB8 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

   Plasmid

   #138525

   Purpose

   Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

   Depositor

   Richard Jenner

   Article

   Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

   Insert

   Control non-PRC2 binding A-tract repeat RNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBactin-Nedd1-mNeonGreen

   Plasmid

   #196864

   Purpose

   Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mNeonGreen (Nedd1 Rat)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS-mTagRFP-TUBA1B

   Plasmid

   #62850

   Purpose

   Donor vector for gene editing at human TUBA1B locus

   Depositor

   Gaudenz Danuser

   Article

   Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

   Insert

   mTagRFP-TUBA1B (TUBA1B Human, Synthetic)
   

   Use

   Yeast-e.coli shuttle vector

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJ4M/TDP-43 S48E

   Plasmid

   #104481

   Purpose

   bacterial expression of TDP-43 S48E

   Depositor

   Nicolas Fawzi

   Article

   Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   MBP-6xHis

   Expression

   Bacterial

   Mutation

   S48E

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBacMam2-DiEx-LIC-C entry vector

   Plasmid

   #111752

   Purpose

   Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with C-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

   Plasmid

   #79887

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Expression

   Bacterial

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)

    Plasmid

    #140893

    Purpose

    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel

    Depositor

    Yasushi Okamura

    Article

    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

    Insert

    CiDr-VSP L223F/C302S-mCherry (vsp, tpte Zebrafish, Ciona intestinalis)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    changed 223L to F in Dr-VSP (zebrafish TPTE), cha…

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

    Plasmid

    #138524

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    PRC2-binding G-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLJM1 Flag GFP 4E-BP1 Rheb15

    Plasmid

    #112761

    Purpose

    express GFP-4EBP1-Rheb15

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag eGFP and Rheb15

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX131

    Plasmid

    #167149

    Purpose

    MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    4'-phosphopantetheinyl transferase, npgA
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PCRII apoEb

    Plasmid

    #121901

    Purpose

    Plasmid for making zebrafish apoEb in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    Apoeb (apoeb Zebrafish)
    

    Use

    Unspecified

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Frt-V5-hspB2

    Plasmid

    #63103

    Purpose

    Expression of HSPB2 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb2 (HSPB2 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. TDP-43_NTD1-80_Y4R

    Plasmid

    #104478

    Purpose

    bacterial expression of TDP-43 NTD Y4R

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y4R

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. psicheck-2-LPPRC-mod

    Plasmid

    #48168

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    LRPPRC leucine rich pentatricopeptide repeat containing (LRPPRC Human)
    

    Use

    Luciferase

    Mutation

    A/T mutation in the ATG of the uORF

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

    Plasmid

    #124278

    Purpose

    Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

    Depositor

    Arthur Laganowsky

    Article

    Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

    Insert

    GIRK2 (Kcnj6 Mouse)
    

    Tags

    eGFP-StreptagII-6xHis

    Expression

    Yeast

    Mutation

    R201A

    Promoter

    AOX1

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST27-GST-ATXN1 FL(92Q)-S776D

    Plasmid

    #21757

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 92 Q(Glutamines) and…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

20. Frt-hspB2

    Plasmid

    #63093

    Purpose

    Expression of HSPB2 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb2 (HSPB2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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