We narrowed to 11,647 results for: emb

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pUPD2_miniDFR (GB2566)

Plasmid

#160595

Purpose

Minimal promoter of slDFR gene containing 62bp upstream the transcription start site and the 5'UTR region

Depositor

Diego Orzaez

Publication

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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px335-EF-Slc2a3-R

Plasmid

#122305

Purpose

Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Slc2a3 (Slc2a3 Synthetic)

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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CS51

Plasmid

#117433

Purpose

Expression of ARL3

Depositor

Richard Kahn

Publication

Cavenagh et al J Biol Chem. 1994 Jul 22;269(29):18937-4

Insert

ARL3 (ARL3 Human)

Expression

Bacterial

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO PP1gamma-2*Strep

Plasmid

#113506

Purpose

Expresses dual Strep-tagged PP1gamma in mammalian cells

Depositor

Hemmo Meyer

Publication

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

PP1gamma (PPP1CC Human)

Tags

Strep-Strep

Expression

Mammalian

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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LI F-G Kan

Plasmid

#104523

Purpose

LI resistance gene module

Depositor

Thomas Lahaye

Publication

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Kanamycin resistance gene

Use

Cloning vector

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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KG#470

Plasmid

#110930

Purpose

Expresses proteins pan-neuronally in C. elegans (entire nervous system)

Depositor

Kenneth Miller

Publication

Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

Inserts

rab-3 promoter
unc-54 3' control region

Expression

Bacterial

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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GST-PglF130

Plasmid

#89708

Purpose

Expresses truncated PglF from C. jejuni with N-terminal GST tag

Depositor

Barbara Imperiali

Publication

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

pglF

Tags

GST

Expression

Bacterial

Mutation

residues 1-129 of putative transmembrane domain h…

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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WPXL SOX10 gRNA

Plasmid

#101923

Purpose

SOX10 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

Depositor

Danwei Huangfu

Publication

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

SOX10 promoter targeting gRNA

Use

Lentiviral

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-Hook3 aa 1-160

Plasmid

#74607

Purpose

Expresses Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Publication

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pUPD_OsU3 (GB1184)

Plasmid

#68209

Purpose

Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

OsU3 promoter

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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p6xUAS (GB0179)

Plasmid

#68182

Purpose

Provides 6xUAS (upstream activating sequence) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

6xUAS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TFDP3

Plasmid

#67083

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Publication

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP3 (TFDP3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pT35S (GB0036)

Plasmid

#68187

Purpose

Provides the CaMV 35S terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

CaMV 35S terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYFP (GB0053)

Plasmid

#68197

Purpose

Provides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

YFP coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-TLEr

Plasmid

#61188

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-hPMCA4bct125

Plasmid

#47594

Depositor

Emanuel Strehler

Insert

PMCA4bct125 (ATP2B4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

125 C terminal AA deleted

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
TAL2418

Plasmid

#36819

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PMS2-TALEN_Left (Pms2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
TAL2419

Plasmid

#36820

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PMS2-TALEN_Right (Pms2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223-CIB4

Plasmid

#23386

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CIB4 (CIB4 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCS2 Antivin

Plasmid

#24961

Depositor

Edward De Robertis

Publication

Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

Insert

Antivin (lefty-a Frog)

Expression

Mammalian

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only