We narrowed to 7,105 results for: mme
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Plasmid#164975PurposeLentiviral vector for NPC1 expression in mammalian cellsDepositorInsertNPC1 (NPC1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationD786N, insertion L aa15 (please see depositor co…PromoterEF1aAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-101-3p
Plasmid#103165PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-101-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-101-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
D28 Cortactin GFP
Plasmid#26723DepositorInsertCortactin (Cttn Mouse)
UseTagsEGFPExpressionMammalianMutationDeletion removed 28 amino acids (aa# 334-361) enc…PromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-hDcp2 E147/8Q
Plasmid#72217Purposebacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorInsertDcp2 (DCP2 Human)
UseTagsGSTExpressionBacterialMutationE147Q, E148Q and F404L (please see depositor'…PromotertacAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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LSB-hsa-miR-516b-5p
Plasmid#103601PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-516b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-516b-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-GPT2-3xFLAG
Plasmid#163449Purposelentiviral overexpression vector for human GPT2DepositorInsertGPT2 (GPT2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCapV
Plasmid#183028Purposethe patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28DepositorInsertpatatin-like phospholipase CapV
UseTagsNoExpressionBacterialMutationPromoterpBAD promoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains1
Plasmid#78461PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2149-2170; Q2513R (please see…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-GPT2-3xFLAG
Plasmid#163447Purposelentiviral overexpression vector for human GPT2DepositorInsertGPT2 (GPT2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-M2rtTA-T2A-Puro
Plasmid#169175PurposepiggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174DepositorInsertM2rtTA-T2A-Puro
UsePiggybac transposonTagsExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-138-5p
Plasmid#103233PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-138-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-138-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-iRFP670 L1-R5
Plasmid#84390PurposeMultisite Gateway entry vector for expression of iRFP670-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsiRFP670ExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-USP13/5-6
Plasmid#61748Purposeexpress N-terminally FLAG tagged human chimera USP13/5-6DepositorUseTagsFLAGExpressionMammalianMutationUSP13 (1-721aa) fused to USP5 (717-859aa); D23Y i…PromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-USP13/5-5
Plasmid#61747Purposeexpress N-terminally FLAG tagged human chimera USP13/5-5DepositorUseTagsFLAGExpressionMammalianMutationUSP5 (1-781aa) fused to USP13 (786-864aa); G2667A…PromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-19b-3p
Plasmid#103327PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-19b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-19b-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-HS-NLRC4Δcard
Plasmid#177276PurposePurification of mouse NLRC4 (90-1024) from insect cellsDepositorInsertNLRC4 (Nlrc4 Mouse)
UseTagsHIS-SUMO-PreScissionExpressionInsectMutationI586T, N761S, M885V, K915R and F972L- please see …PromoterpolyhedrinAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEntr1a-mcherry-FKBP-TIAM1(DHPH)
Plasmid#176135PurposeHeterodimerization with FRB (rapamycin), Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
UseEntry vectorTagsExpressionMutationSee Depositor Comments BelowPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7f-5p
Plasmid#103156PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7f-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7f-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-133a-3p
Plasmid#103221PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-133a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-133a-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only