We narrowed to 7,201 results for: mme

1. MSCV-Scara3-IRES-BFP

   Plasmid

   #117265

   Purpose

   Retroviral overexpression of Scara3

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   Scara3 (Scara3 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   See depositor comments below.

   Promoter

   LTR

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-At_PRORP1-His

   Plasmid

   #67869

   Purpose

   bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

   Depositor

   Walter Rossmanith

   Article

   Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

   Insert

   PRORP1 (PRORP1 Mustard Weed)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Residues 36-572 (see comments)

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. M72-IRES-tauRFP2-ACNF TV

   Plasmid

   #15512

   Depositor

   Peter Mombaerts

   Article

   Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

   Insert

   M72-IRES-tauRFP2-ACNF TV (Olfr160 Mouse)
   

   Use

   Mouse Targeting

   Mutation

   Targeting vector to insert IRES-tauRFP2-ACNF imme…

   Available Since

   Aug. 24, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-Ro2

   Plasmid

   #24503

   Depositor

   Bruce Conklin

   Article

   Coward et al Proc Natl Acad Sci U S A. 1998 Jan 6;95(

   Insert

   RASSL Ro2 (OPRK1 Human)
   

   Tags

   FLAG and NLS from prolactin

   Expression

   Mammalian

   Mutation

   mutations in extracellular loops 2 (see comments …

   Available Since

   June 16, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

   Plasmid

   #127308

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTV-mHdac1-tdiRFP670-PEST2

   Plasmid

   #122351

   Purpose

   Targeting vector for mouse Hdac1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   2A-tdiRFP-PEST with homology arms for Hdac1
   

   Use

   Mouse Targeting

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDUAL-P41nmt1-Nup124-GFP-MTn

   Plasmid

   #154365

   Purpose

   Yeast expression of Nup124

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   Nup124 (nup124 Fission Yeast)
   

   Expression

   Yeast

   Mutation

   See depositor comments below

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTV-Met-Eif4a1-tdiRFP670-PEST

   Plasmid

   #122371

   Purpose

   Targeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon.

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   2A-tdiRFP-PEST-2A with homology arms for Eif4a1
   

   Use

   Mouse Targeting

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. M26I DJ-1 TEV site pET15b

   Plasmid

   #60685

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   M26I DJ-1 (PARK7 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M26I and TEV site was inserted (see comments belo…

   Promoter

   T7

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

10. M26L DJ-1 TEV site pET15b

    Plasmid

    #60686

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26L DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26L and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

11. EGFP_EHMT1B1-4

    Plasmid

    #228812

    Purpose

    Expression of mouse EHMT1 with deletion of aa 809-1298

    Depositor

    Shravanti Rampalli

    Article

    Biligiri et al PLOS Biology 22(11): e3002871.

    Insert

    EHMT1 (Ehmt1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of aa 809-1298, See Depositor Comments

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-nucGFP11-3xPax7gRNA

    Plasmid

    #224570

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    GFP11-H2B
    

    Use

    CRISPR

    Tags

    Histone H2B (nuclear localization)

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-3xGFP11-mem-3xPax7gRNA

    Plasmid

    #224571

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEYFP_C1_ATRX

    Plasmid

    #221383

    Purpose

    Expresses ATRX in mammalian cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Transcriptional regulator ATRX (ATRX Human)
    

    Expression

    Mammalian

    Mutation

    K536N (Please see depositor comments)

    Promoter

    CMV

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-VcnTS-E1015A

    Plasmid

    #213415

    Purpose

    Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnTS-E1015A (VCL Chicken, Synthetic)
    

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 to alanine (E…

    Promoter

    CMV

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-VcnTS-E1021A

    Plasmid

    #213416

    Purpose

    Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnTS-E1021A (VCL Chicken, Synthetic)
    

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1021 to alanine (E…

    Promoter

    CMV

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-VcnCS-E1015A

    Plasmid

    #213412

    Purpose

    Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnCS-E1015A (VCL Chicken, Synthetic)
    

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 to alanine (E…

    Promoter

    CMV

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-VcnCS-E1021A

    Plasmid

    #213413

    Purpose

    Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnCS-E1021A (VCL Chicken, Synthetic)
    

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1021 to alanine (E…

    Promoter

    CMV

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPBpuro-UbC-canisEgf

    Plasmid

    #173845

    Purpose

    Encoding dog Egf.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A cDNA of canis EGF.
    

    Expression

    Mammalian

    Mutation

    R1127Q- please see dep. comments

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-FKBP-E2

    Plasmid

    #175243

    Purpose

    Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBE2I (UBE2I Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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