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We narrowed to 560 results for: mme

Showing: 1 - 20 of 560 results

  1. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...The NeuroMab Facility and James Trimmer have produced monoclonal antibodies validated for neuroscience...Collection NeuroMab Plasmid Collection NeuroMab/Trimmer Lab Plasmid Collection R-mAb Plasmids scFv Plasmids...the laboratory of NeuroMab Director, Dr. James Trimmer, have produced numerous monoclonal antibodies (...the culture media. For high level expression we recommend using the HEK-2936E cell line engineered by Durocher...e9. PMID: 11788735 (Link opens in a new window) Trimmer J, et al. 2020. Recombinant Antibodies in Basic...

  2. Summer SciComm: Preprints

    Type
    Blog Post
    ...review or curation models where editors’ comments, reviewers’ comments, and author responses can be shared ... the Addgene blog Summer SciComm Series: Modes of Communication Intro to Our Summer SciComm Series!...these issues, by sharing their work publicly and immediately once the manuscript is drafted. Preprints were...for authors to post. They can be assessed and commented on by readers, typically peers in the field, and...on your poster, or invite attendees to read and comment online! Want the general public (say, policymakers...Preprints and their feedback, in contrast, are immediately and openly accessible, allowing problematic work...

  3. Summer Fun at Addgene!

    Type
    Blog Post
    ...launch this summer! Summer SciComm Series From June-September, we’ll be running a Summer Science Communication... is finally warming up in Boston and that means summer is right around the corner. Here at Addgene, we...inspired by the new seasons, so we've made some big summer plans this year! New Blog Series We have not one...soon! We’ll also be building our video library all summer long, with additions to our other video series.... and warm weather - check out our Instagram all summer long to see what kind of shenanigans we get into...

  4. An Addgene Summer Internship

    Type
    Blog Post
    ... for summer programs. I spent one day at Addgene last summer, through the La Vida Scholars summer program...Figure 1: Addgene's summer interns presenting their capstone projects during the Summer Intern Poster Presentation...I never would have imagined that my summer before high school senior year would have been spent within...Addgene’s internship opportunity taking place next summer.  I applied to Addgene and began the interview...position, but I was waiting to hear back from other summer programs. Amy took into consideration just how ...with exciting news. I managed to secure a virtual summer program, teaching me the basics of physics and ... senior in high school who just completed their summer internship at Addgene. They plan on majoring in...

  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...anti-GFAP [N206A/8R] antibody shared by James Trimmer’s lab (Figure 5) as well as antibodies for identifying...N206A/8 TC Supernatant). Image attribution: James Trimmer, UC Davis.   ...

  6. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...Welcome to the last of our Summer SciComm Series! We hope you've enjoyed this small glimpse into the ...SSCS: Cognitive Load SSCS: Preprints Intro to our Summer SciComm Series  ...

  7. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...resources on the Addgene blog Summer SciComm Series: Cognitive LoadSummer SciComm Series: Modes of CommunicationIntro...courtesy of Leonardo Ramos.  I enrolled into a summer school course at the University of Otago’s Department...CommunicationIntro to Our Summer SciComm Series!...

  8. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ... on the Addgene blog:  Summer SciComm Series: Cognitive LoadIntro to our Summer SciComm Series    ...high Uses visuals, audios, and text. Incredibly immersive and flexibility to visualize and contextualize...

  9. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...We are now at the second post in our Summer SciComm series and it’s time for one of my favorite scicomm...immunologist, a geneticist, a physicist, etc., is an immense task (it takes, ooh, approximately the length of... about the questions you’ve received, read the comments, seek feedback from others, and listen quietly...More resources on the Addgene blog Intro to our Summer SciComm Series...

  10. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...Welcome to our Summer SciComm Series, where we’ll be talking about science communication, best practices... active science communication research.   This summer, we’re making time to talk about my favorite thing...scicomm)! We have so many fun topics planned for this summer, but the foundation of all good communication is...

  11. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...reading your material. For the vast majority of summer internships you are applying to, keep your resume... a solid personal statement.  4. Letters of recommendation wisdom  Reach out to 2-3 individuals in your...personal statement. Make certain to choose your recommenders based on your relationships to the person as...of influence. In other words, the longer the recommender knows you the better, and their leadership status...statement along with the request to help your recommenders deliver a superb and detailed portrayal of yourself...

  13. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...now in the summer of 2020 that the consequences of undermining science are even more immediate and dire ... Marc Zimmer’s recent book, The State of Science: What the Future Holds and the Scientists Making It ... “What is science?” That’s a very big question. Zimmer, a Professor of Chemistry at Connecticut College...If that sounds ambitious, that’s because it is. Zimmer, whose previous books focused primarily on fluorescent...nearly impossible for a career scientist such as Zimmer to introduce any one of these topics without some...hobbies, are unlikely to be surprised by many of Zimmer’s revelations. The issues he discusses have been...understand every other problem. For non-scientists, Zimmer’s observations will likely range from mildly surprising...

  15. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...double-stranded break repair mechanism: MMEJ (microhomology-mediated end-joining)? MMEJ, a form of alternative end-...balance of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect ...stranded breaks are repaired by two rounds of MMEJ. This double MMEJ strategy looks very similar to HDR, but... possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (2016).  “MMEJ-assisted gene knock-in...Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new method for CRISPR gene knock-in,...need for complicated cloning of homology arms. MMEJ: An Introduction There are three primary methods...with insertions and deletions common. In contrast, MMEJ uses regions with 5-25 bp of microhomology flanking...

  17. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...levels and transport (FLIPglu) Frommer Lab FLIPglu Plasmids Wolf Frommer Glucose FRET sensor to monitor...levels and transport (FLIIglu) Frommer Lab FLIIglu Plasmids Wolf Frommer Glucose Green fluorescent glucose...transport and metabolism (FLIPE) Frommer Lab FLIPE Plasmids Wolf Frommer Glutamate Ultrafast glutamate sensors...proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer Calcium Red-, yellow-, or cyan-emitting homoFRET...using optical sensors. Biochem J. 2010 Dec 1. Wolf Frommer ATP yAT1.03 FRET-based ATP sensor for yeast An ...FRET-based biosensor for cyclic-di-GMP measurement Asymmetrical distribution of the second messenger c-di-GMP...Biotechnol Biofuels. 2008 Jun 3. 1(1):11. Wolf Frommer Maltose Green fluorescent MBP-based maltose biosensor...

  18. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...James Trimmer 177520 Anti-LRRK2/Dardarin, C-terminus [N241A/34R] LRRK2 CMV Parkinson's James Trimmer 177537...Spinocerebellar ataxia James Trimmer 177546 Anti-PINK1 [N4/15R] PINK1 CMV Parkinson's James Trimmer 177653 pT7II-2N4Rtau...James Trimmer 182104 Anti-LRRK2/Dardarin, N-terminus [N138/6R] LRRK2 CMV Parkinson's James Trimmer 182113...Spinocerebellar ataxia 19 James Trimmer 188163 Anti-VAPA/B [N479/107R] VAPB CMV ALS James Trimmer 188167 Anti-IP3 receptor...ataxia James Trimmer 196946 Kv4.3 long/RBG4 KCND3 CMV Spinocerebellar ataxia 19 James Trimmer 197107 iYFP-Parkin...19 James Trimmer 206543 Anti-LRRK2/Dardarin, N3 [N231B/34R] LRRK2 CMV Parkinson's James Trimmer 206623 ... James Trimmer 206681 Anti-Kv1.1 K+ channel [K20/78R-1] KCNA1 CMV Episodic ataxia James Trimmer 206684...

  19. Antibody Production

    Type
    Collection
    ...Production & Usage” and “Delivery” conditions, so we recommend reviewing the specific antibody webpage for further...across a tissue panel in one of the antibody’s recommended applications. The specific QC experiments performed...Storage and Shipping Stability Addgene does not recommend repeated freeze thaw of antibodies and suggests... against a non-treated control in one of its recommended assays. If an antibody has passed stability testing...

  20. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...genomic target, you need to make sure the NGG immediately follows your target on the genome but NOT on ...considerations only matter if the PAM sequence is immediately adjacent to the nearly-homologous target sites...work, do you have any suggestions? The Zhang lab recommends using the Epicentre QuickExtract solution to ... the second design (in pX330 / PX335 ), so we recommend people all use the pX330/PX335 system ....
Showing: 1 - 20 of 560 results