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Showing: 1 - 20 of 486 results

  1. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ... for summer programs. I spent one day at Addgene last summer, through the La Vida Scholars summer program...Figure 1: Addgene's summer interns presenting their capstone projects during the Summer Intern Poster Presentation...I never would have imagined that my summer before high school senior year would have been spent within...Addgene’s internship opportunity taking place next summer.  I applied to Addgene and began the interview...position, but I was waiting to hear back from other summer programs. Amy took into consideration just how ...with exciting news. I managed to secure a virtual summer program, teaching me the basics of physics and ... senior in high school who just completed their summer internship at Addgene. They plan on majoring in...

  2. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...review or curation models where editors’ comments, reviewers’ comments, and author responses can be shared ... the Addgene blog Summer SciComm Series: Modes of Communication Intro to Our Summer SciComm Series!...these issues, by sharing their work publicly and immediately once the manuscript is drafted. Preprints were...for authors to post. They can be assessed and commented on by readers, typically peers in the field, and...on your poster, or invite attendees to read and comment online! Want the general public (say, policymakers...Preprints and their feedback, in contrast, are immediately and openly accessible, allowing problematic work...

  3. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...launch this summer! Summer SciComm Series From June-September, we’ll be running a Summer Science Communication... is finally warming up in Boston and that means summer is right around the corner. Here at Addgene, we...inspired by the new seasons, so we've made some big summer plans this year! New Blog Series We have not one...soon! We’ll also be building our video library all summer long, with additions to our other video series.... and warm weather - check out our Instagram all summer long to see what kind of shenanigans we get into...

  4. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...anti-GFAP [N206A/8R] antibody shared by James Trimmer’s lab (Figure 5) as well as antibodies for identifying...N206A/8 TC Supernatant). Image attribution: James Trimmer, UC Davis.   ...

  5. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...Welcome to the last of our Summer SciComm Series! We hope you've enjoyed this small glimpse into the ...SSCS: Cognitive Load SSCS: Preprints Intro to our Summer SciComm Series  ...

  7. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...resources on the Addgene blog Summer SciComm Series: Cognitive LoadSummer SciComm Series: Modes of CommunicationIntro...courtesy of Leonardo Ramos.  I enrolled into a summer school course at the University of Otago’s Department...CommunicationIntro to Our Summer SciComm Series!...

  8. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ... on the Addgene blog:  Summer SciComm Series: Cognitive LoadIntro to our Summer SciComm Series    ...high Uses visuals, audios, and text. Incredibly immersive and flexibility to visualize and contextualize...

  9. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...We are now at the second post in our Summer SciComm series and it’s time for one of my favorite scicomm...immunologist, a geneticist, a physicist, etc., is an immense task (it takes, ooh, approximately the length of... about the questions you’ve received, read the comments, seek feedback from others, and listen quietly...More resources on the Addgene blog Intro to our Summer SciComm Series...

  10. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...Welcome to our Summer SciComm Series, where we’ll be talking about science communication, best practices... active science communication research.   This summer, we’re making time to talk about my favorite thing...scicomm)! We have so many fun topics planned for this summer, but the foundation of all good communication is...

  11. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...reading your material. For the vast majority of summer internships you are applying to, keep your resume... a solid personal statement.  4. Letters of recommendation wisdom  Reach out to 2-3 individuals in your...personal statement. Make certain to choose your recommenders based on your relationships to the person as...of influence. In other words, the longer the recommender knows you the better, and their leadership status...statement along with the request to help your recommenders deliver a superb and detailed portrayal of yourself...

  12. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...now in the summer of 2020 that the consequences of undermining science are even more immediate and dire ... Marc Zimmer’s recent book, The State of Science: What the Future Holds and the Scientists Making It ... “What is science?” That’s a very big question. Zimmer, a Professor of Chemistry at Connecticut College...If that sounds ambitious, that’s because it is. Zimmer, whose previous books focused primarily on fluorescent...nearly impossible for a career scientist such as Zimmer to introduce any one of these topics without some...hobbies, are unlikely to be surprised by many of Zimmer’s revelations. The issues he discusses have been...understand every other problem. For non-scientists, Zimmer’s observations will likely range from mildly surprising...

  14. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...double-stranded break repair mechanism: MMEJ (microhomology-mediated end-joining)? MMEJ, a form of alternative end-...balance of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect ...stranded breaks are repaired by two rounds of MMEJ. This double MMEJ strategy looks very similar to HDR, but... possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (2016).  “MMEJ-assisted gene knock-in...Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new method for CRISPR gene knock-in,...need for complicated cloning of homology arms. MMEJ: An Introduction There are three primary methods...with insertions and deletions common. In contrast, MMEJ uses regions with 5-25 bp of microhomology flanking...

  16. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...validation of some of these tools created by the Trimmer Lab that will be the first antibodies distributed...constructing the plasmids for expression of mAbs, the Trimmer lab isolated RNA from cryopreserved hybridomas,...can be a threat to future reproducibility. The Trimmer Lab found that in addition to recovering RNA from...lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation by immunofluorescence-immunocytochemistry...subsequently cloned into the expression constructs. The Trimmer lab found that the recognition sequence for the...webpage. Importantly, the methods developed by the Trimmer Lab for construction and validation of R-mAb expressing...with different mAbs in the same experiment. The Trimmer Lab found that they could modify the CH encoding...

  17. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...cases”. Cases present specific operational or commercial challenges or questions that clients face imminently... and help resolve it through well-informed recommendations. Healthcare consulting firms that specialize... health and economic benefits of novel drugs. Commercial Affairs teams make up a large and diverse group...group of professionals running the marketing, commercialization, collaborative, and financial functions associated...associated with the business. Commercial teams routinely work with consulting firms on projects such as... conversations with R&D, Medical Affairs, and Commercial teams, reflecting the need for all parts of a...crucial for the success of a drug’s clinical and commercial future, and yet are incredibly difficult to find...

  18. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...version of the plasmid (Summers & Sherratt, 1984; Summers et al., 1993; Summers, 1998). Multimeric plasmids... replicate at a higher frequency (Summers & Sherratt, 1984; Summers et al., 1993). These larger plasmids...monomers and various forms of multimers (Summers & Sharratt, 1984; Summers et al., 1993). In these cases, screening...strategies for resolving multimers? Let us know in the comments below or contact us at [email protected]. Resources...electrophoresis. BioProcess Int. 2006 Sep;4(8):38-40. Summers D. Timing, self-control and a sense of direction....1046/j.1365-2958.1998.01012.x. PMID: 9767582. Summers DK, Beton CW, Withers HL. Multicopy plasmid instability...10.1111/j.1365-2958.1993.tb01648.x. PMID: 8361350. Summers DK, Sherratt DJ. Multimerization of high copy number...

  19. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...Many antibodies will have a recommended blocking buffer and a recommended concentration. That makes the...particularly your primary antibodies. We always recommend using an antibody validated for your target in... are different than the validation assays, we recommend performing your own validation assays. If you’...There are many different lysis buffers available commercially, and most common buffers have recipes available...to preserve specific protein modifications, we recommend doing a little more research into gel chemistry...separation of proteins of different sizes.  Table 2: Recommended gel percentages for various protein sizes ...binding and washes. It’s less sturdy and not recommended for stripping and reprobing, unless you buy specialty...

  20. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ... is my experience looking for the perfect job. Summer work experience in cancer research After graduating...would be very competitive, and so I applied for a summer role as a Research Scientist at the Institute of...to gain relevant experience. I applied for this summer position as I felt I needed more practical lab ...study of e2Fb, which is a tumour suppressor. This summer role was incredibly valuable because it was a set... need to explain why I changed jobs. I highly recommend taking on a similar position in your field of ...and enjoyed them, so I applied for a role at a commercial virology testing company. This particular company...companies. It was really exciting to work in a larger commercial company; the pay was good and there was a variety...
Showing: 1 - 20 of 486 results