We narrowed to 16,043 results for: GRN

1. pDECKO_TFRC_C (with mCherry)

   Plasmid

   #72627

   Purpose

   Expresses two gRNAs targeting the TFRC promoter

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward TFRC
   

   Expression

   Mammalian

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUDP046

   Plasmid

   #107062

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting KU80 from Ogataea parapolymorpha

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting OpKU80 inO. parapolymorpha
   

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUDP082

   Plasmid

   #103875

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUDP025

   Plasmid

   #103874

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUDP013

   Plasmid

   #103873

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1)

   Plasmid

   #217345

   Purpose

   crRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Inserts

   crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
   crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
   crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
   crCLTA-4 gRNA: ggctctgcaacaccgcctagacc (CLTA Human)
   crFOLH1-1 gRNA: gctccagacctggggtccagttt (FOLH1 Human)
   crCD151-3 gRNA: cgggaggccgcacccaccgcctg (CD151 Human)
   crHBG-3 gRNA: ttcttcatccctagccagccgcc (HBG1, HBG2 Human)
   crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
   crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
   crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFD6

   Plasmid

   #73915

   Purpose

   tissue-specific multiplex gRNA plasmid

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   :tRNA:gRNA:tRNA:gRNA:tRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   UAS-DSCP

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCJ1022

   Plasmid

   #228762

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Pkd2 gRNAs (Pkd2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCJ1021

   Plasmid

   #228761

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Ift88 gRNAs (Ift88 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAH750

    Plasmid

    #91212

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters, structurally optimized gRNA scaffolds)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEE491

    Plasmid

    #149292

    Purpose

    T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEE531

    Plasmid

    #149290

    Purpose

    T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEE495

    Plasmid

    #149291

    Purpose

    T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSG4K5

    Plasmid

    #74492

    Purpose

    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTR(EF)-tRNA(Q)

    Plasmid

    #170517

    Purpose

    PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Insert

    gRNAcore(EF)-tRNA(Q)
    

    Use

    Pcr template vector for amplifying grnacore(ef)-t…

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHSG1C3

    Plasmid

    #164423

    Purpose

    single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCJ1023

    Plasmid

    #228763

    Purpose

    Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pALuWBB

    Plasmid

    #183202

    Purpose

    dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA

    Depositor

    Mitch McVey

    Insert

    w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
    

    Use

    CRISPR; Recombines at attp landing site in fly ge…

    Expression

    Insect

    Promoter

    U6-3

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFD5

    Plasmid

    #73914

    Purpose

    multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    dU6-3:tRNA:gRNA:tRNA:gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6:3

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWT050d

    Plasmid

    #107901

    Purpose

    W2.5-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA6-(sgRNA4-C16T-C18T) and is part of CAMERA 2.5

    Depositor

    David Liu

    Article

    Tang et al Science. 2018 Feb 15. pii: science.aap89

    Insert

    PLacO-sgRNA1, PRha-sgRNA6-(sgRNA4-C16T-C18T)
    

    Expression

    Bacterial

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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