We narrowed to 11,284 results for: VARS

1. pAH33

   Plasmid

   #226850

   Purpose

   To constitutively overexpress Opa1 from the safe harbor locus Rosa26 in MEF

   Depositor

   Luke Chao

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   Opa1 (Opa1 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOGS539_GLuc

   Plasmid

   #226718

   Purpose

   Plasmid enabling yeast-mediated expression and secretion of Gaussia luciferase (GLuc)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Gaussia Luciferase
   

   Tags

   HA tag and Mating factor alpha secretion signal

   Expression

   Yeast

   Promoter

   pTEF1

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)

   Plasmid

   #226989

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 1a to create OPA1-del_exon5
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

   Plasmid

   #226991

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 2a to create OPA1-del_exon5
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A6-gRNA_(CJT88)

   Plasmid

   #226987

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A6)

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA A6 to create OPA1-R194G
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A7-gRNA_(CJT89)

   Plasmid

   #226986

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A7)

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA A7 to create OPA1-R194G
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

   Plasmid

   #226992

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 2b to create OPA1-del_exon5
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

   Plasmid

   #226990

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 1b to create OPA1-del_exon5
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A9-gRNA_(CJT87)

   Plasmid

   #226988

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A9)

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA A9 to create OPA1-R194G
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG8Ha-His-EWSR1-359-549

    Plasmid

    #195868

    Purpose

    Expresses the RRM-RG2-ZnF (359-549) of EWS

    Depositor

    David Libich

    Article

    Selig et al Biopolymers. 2023 May;114(5):e23536. doi

    Insert

    EWS 359-549 (EWSR1 Human)
    

    Tags

    His8-linker-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. macroH2A1.1-F192V-GFP (pc5114)

    Plasmid

    #223437

    Purpose

    Mutation phenylalanine 192 to valine (F192V) in macroH2A1.1-GFP

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

    Insert

    macroH2A1.1-F192V (Macroh2a1 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti HsATP13A2 P652_E653 del

    Plasmid

    #213700

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP13A2 P652_E653 del

    Depositor

    Veerle Baekelandt

    Article

    Colijn et al Neurogenetics. 2024 Jul 18. doi: 10.1007

    Insert

    human ATP13A2 P652_E653 del (ATP13A2 Human)
    

    Use

    Lentiviral

    Mutation

    WT, D962N

    Promoter

    CMV

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPINVHH_A8_His

    Plasmid

    #201718

    Purpose

    Expression of nanobody

    Depositor

    Ray Owens

    Article

    Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

    Insert

    VHH_A8 (S lama glama (llama))
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPINVHH_A10-5_His

    Plasmid

    #201720

    Purpose

    Expression of nanobody

    Depositor

    Ray Owens

    Article

    Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

    Insert

    VHH_A10-5 (S lama glama (llama))
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPINVHH_B5-5_His

    Plasmid

    #201721

    Purpose

    Expression of nanobody

    Depositor

    Ray Owens

    Article

    Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

    Insert

    VHH B5-5 (S lama glama (llama))
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-enCas12a-A3A-N57G

    Plasmid

    #193651

    Purpose

    Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLb-enCas12a-hA3A-N57G, EGFP
    

    Expression

    Mammalian

    Mutation

    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV6-AN-DDK_S172A_M173A_V195A RAD23A

    Plasmid

    #201576

    Purpose

    Expresses a variant of human RAD23A containing mutations S172A, M173A and V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutations S172A, M173A and V195A

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Stx5L(m55V)-GFP

    Plasmid

    #209845

    Purpose

    Expresses Stx5 M55V mutant fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    STX5 (STX5 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Methionine 55 to Valine, causes loss of S…

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. B-HypaR-SpCas9

    Plasmid

    #126764

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Hypa2SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A; N692A; M694A; Q695A; H698A; amino acids 10…

    Promoter

    CBh

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. ERAP1-rs27895(C)

    Plasmid

    #206142

    Purpose

    Expresses the rs27895-C allele of ERAP1, myc-tagged.

    Depositor

    Dennis Ko

    Article

    Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

    Insert

    ERAP1 (ERAP1 Human)
    

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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