We narrowed to 13,228 results for: sequence
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Plasmid#193629PurposeConstitutive T7 RNAP-mediated expression of LasR transcription factor for Las quorum sensing system. LasR sequence is codon optimized for E. coli.DepositorInsertLasR
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
phRL TK 5BoxB sp 36
Plasmid#115365PurposeExpression vector to produce humanized Renilla luciferase with 5BoxB sequences in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseExpressionMammalianPromoterTKAvailable SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT
Plasmid#19291DepositorInsertE-cadherin
UseLuciferaseExpressionMammalianMutationE-cad promoter sequences from -108 to +125 Ebo…Available SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBAD_IPF2.0
Plasmid#163124PurposeA plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.DepositorInsertIFP2.0
TagsHisExpressionBacterialAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B1-deltaE1-MCS
Plasmid#122558PurposeModified block 1, with deletion in E1DepositorInsertAdenovirus 5 genomic region 1-3759
UseSynthetic BiologyMutationAdenovirus sequences 481-3530 replaced with multi…Available SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS_36x-601_MA1+MA3+MA2
Plasmid#114364Purpose36x Widom 601 nucleosome sequenceDepositorInsert36X601-MA1_3_2
UseUnspecifiedExpressionMammalianAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gata4-GFP donor
Plasmid#113115PurposeDonor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequenceDepositorInsertGata4 donor region including homology arms and eGFP
UseSynthetic BiologyAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4282
Plasmid#98701PurposeA plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF mito-cGFP (matrix)
Plasmid#188904PurposeExpresses mitochondrial GFPDepositorInsertmitochondrial GFP
TagsMitochondrial leader sequence: MSVLTPLLLRGLTGSARR…ExpressionMammalianAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B6-deltaE3
Plasmid#122559PurposeModified block 6, with deletion in E3DepositorInsertAdenovirus 5 genomic region 27859-30803
UseSynthetic BiologyMutationAdenovirus sequences 27859-30803 replaced with Ba…Available SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM710
Plasmid#68893PurposeIPTG-inducible CRISPRi vector targeting nonsense sequence (NS), pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
A1-20B1 (P001)
Plasmid#161820PurposeThis plasmid carries Yn situ preamplifier A1-20B1 sequence.DepositorInsertA1-20B1
UseSynthetic BiologyAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-Flp1)
Plasmid#194770PurposeTo insert T2A-Flp1 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Flp1 transgene.DepositorInsertT2A-Flp1
UseCRISPRExpressionInsectAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMH220
Plasmid#122836PurposedCas9 with GreenGate B-C flanking sequencesDepositorInsertNuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
UseGolden gate compatible cloning vectorMutationContains D10A and H840A mutationsAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pet28a-ClbB-NRPS-NHis
Plasmid#49217PurposeNRPS module of ClbB containing CAT domains with N-terminal 6His tagDepositorInsertClbB-NRPS
Tags6x His tag and T7 tagExpressionBacterialMutationcontains aa4-1080 of reference sequence NP_754362…PromoterT7Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBYK01
Plasmid#138656PurposephiOZJ integrating Streptomyces expression vector lacking RDFDepositorTypeEmpty backboneExpressionBacterialPromoterPermE* variant sequence from pDA1652Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiMutB3GNT5-blast
Plasmid#208380Purposelentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequenceDepositorInsertBeta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
UseLentiviralTagsmyc, FLAGExpressionMammalianMutationnt 922 c->g (PAM site inactivation)PromoterEF-1 alpha core promoterAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMD5
Plasmid#138652PurposephiBT1 integrating Streptomyces expression vectorDepositorTypeEmpty backboneExpressionBacterialPromoterPermE* variant sequence from pDA1652Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S
Plasmid#211839PurposePlant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.DepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationR181A R185APromoterUBIQUITIN10Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only