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pHAGE-3FLAG-TFR1

Plasmid

#176490

Purpose

For lentiviral transduction of 3FLAG-TFR1

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

TFR1 (TFRC Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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OPA1-isoform7

Plasmid

#70179

Purpose

Mammalian expression of hsOPA1 isoform 7

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 7 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rubicon

Plasmid

#21636

Depositor

Tamotsu Yoshimori

Article

Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

Insert

Rubicon (1700021K19Rik Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

*(see below)

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

Plasmid

#35500

Purpose

AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E122T and E162T

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

Plasmid

#35501

Purpose

AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128S and D156A

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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OPA1-isoform2

Plasmid

#70174

Purpose

Mammalian expression of hsOPA1 isoform 2

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 2 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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OPA1-isoform3

Plasmid

#70175

Purpose

Mammalian expression of hsOPA1 isoform 3

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 3 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only
OPA1-isoform4

Plasmid

#70176

Purpose

Mammalian expression of hsOPA1 isoform 4

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 4 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
OPA1-isoform6

Plasmid

#70178

Purpose

Mammalian expression of hsOPA1 isoform 6

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 6 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only
pGM190

Plasmid

#69614

Purpose

inducible gene expression in Streptomyces

Depositor

Günther Muth

Article

Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

Inserts

aphII
tsr
rep
sso
TipA promoter
dso

Use

Streptomyces- e. coli shuttle vector

Expression

Bacterial

Available Since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV GFP-GABARAPL1 WT

Plasmid

#123117

Purpose

Expresses EGFP-GABARAPL1 wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3B G120

Plasmid

#123094

Purpose

Expresses 3xFLAG-LC3B G120 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acids 121-125. Stop codon after G12…

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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plenti_DCLK1 G533N

Plasmid

#163627

Purpose

Expresses DCLK1 G533N in E. coli

Depositor

Kenneth Westover

Article

Liu et al Cell Chem Biol. 2020 Oct 15;27(10):1229-

Insert

DCLK1 (DCLK1 Human)

Use

Lentiviral

Tags

V5

Mutation

G33N

Promoter

sv40

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

Plasmid

#124537

Purpose

Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Insert

deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro

Expression

Mammalian

Mutation

d11-41 C55G, d11-41 C55G

Promoter

Minimal ADE

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-GABARAP-GFP

Plasmid

#123111

Purpose

Expresses 3xFLAG-GABARAP-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAP

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3B G120A

Plasmid

#123093

Purpose

Expresses 3xFLAG-LC3B G120A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 120 to Alanine

Promoter

CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-LC3C-GFP

Plasmid

#123110

Purpose

Expresses 3xFLAG-LC3C-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3C

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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plenti_DCLK1 G511N

Plasmid

#163626

Purpose

Expresses DCLK1 G511N in E. coli

Depositor

Kenneth Westover

Article

Liu et al Cell Chem Biol. 2020 Oct 15;27(10):1229-

Insert

DCLK1 (DCLK1 Human)

Use

Lentiviral

Tags

V5

Mutation

G511N

Promoter

sv40

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(D3A: D175A D28A D9A)

Plasmid

#90090

Purpose

Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

Depositor

Clay Clark

Article

Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

D175A D28A D9A

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo-myc-LC3-HA

Plasmid

#45244

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

HA and myc

Expression

Mammalian

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only