Addgene

We narrowed to 4,250 results for: TIL;

Showing: 621 - 640 of 4250 results

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pSR1058

Plasmid

#208743

Purpose

Characterization plasmid for PHbs (sfgfp expressed from PHbs on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PHbs

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PHbs

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pB-Rex

Plasmid

#122134

Purpose

B. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 origin

Depositor

Srivatsan Raman

Article

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

B. subtilis Rex

Use

Tags

Expression

Bacterial

Mutation

Promoter

apFAB338

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-BirA-ER

Plasmid

#20856

Depositor

Alice Ting

Article

Howarth et al Nat Methods. 2008 May . 5(5):397-9.

Insert

BirA-ER

Use

Tags

Expression

Mammalian

Mutation

C-terminal ER targeting sequence (KDEL)

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pET11_Ec_ORF209

Plasmid

#69029

Purpose

Expresses E.coli ORF209/ADPRase in bacterial cells

Depositor

Sandra Gabelli, Maurice Bessman

Article

Gabelli et al Nat Struct Biol. 2001 May;8(5):467-72.

Insert

ORF209/ADPRase

Use

Tags

-

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only
pGGF000

Plasmid

#48861

Purpose

Empty entry vector for creating GreenGate plant resistance cassette modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)

Plasmid

#225752

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCG004

Plasmid

#87377

Purpose

ORF part assembly entry vector derived from pHT01.

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

GFP dropout part

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTU2-AmyE-Pveg-eGFP

Plasmid

#112792

Purpose

Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation

Depositor

Chris French

Article

Wicke et al bioRxiv 185108

Insert

Pveg-eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDR-GW AT1.03

Plasmid

#28003

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03

Use

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pOPINF

Plasmid

#26042

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

His3C-lacZ

Use

Tags

MAHHHHHHSSGLEVL

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pOPINJ

Plasmid

#26045

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

HisGST3C-lacZ

Use

Tags

MAHHHHHH_GST_LEVLFQGT

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)

Plasmid

#225753

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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p5-79

Plasmid

#118087

Purpose

N-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

N-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGGC025

Plasmid

#48830

Purpose

Provides 3xGFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

3xGFP

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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p6-8

Plasmid

#118089

Purpose

rapa-T7- mRNA(GFP)

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

Rapamycin-inducible split T7 RNAP with T7 driven mRNA for GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pVH21-Tier1-PhEF1a

Plasmid

#169561

Purpose

Tier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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p7XNH3

Plasmid

#47064

Purpose

FX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-mYFP-hphMX6

Plasmid

#105155

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu, Thomas Pollard

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mYFP-hphMX6

Use

Tags

mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pNY-351

Plasmid

#231048

Purpose

Used for staining GFP

Depositor

Jeremy Nathans

Insert

anti GFP DARPin fused to goat Fc

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMyNT

Plasmid

#42191

Depositor

Annabel Parret, Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

ColE1

Available since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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