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pIR3DH8

Plasmid

#165068

Purpose

Knocking out GAL80 using KlURA3 marker

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

gal80Arm1-P-AgTEF1-KlURA3-T-AgTEF1-gal80Arm2

Expression

Yeast

Mutation

WT

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Strep II

Plasmid

#153512

Purpose

Strep II C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Strep II

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_TwinStrep

Plasmid

#153513

Purpose

TwinStrep C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

TwinStrep

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pmKaxxte

Plasmid

#113630

Purpose

to test Cas9 gRNA efficiency or HR-capacity

Depositor

Ken Mills

Article

Liberante et al Sci Rep. 2019 Feb 25;9(1):2678. doi: 10.

Insert

mKaxxte2.5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET14b-Prp5

Plasmid

#111347

Purpose

protein purification

Depositor

Christine Guthrie

Insert

PRP5

Tags

6xHis

Expression

Bacterial

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pD864-LacZ backbone

Plasmid

#109384

Purpose

Empty plasmid of the pD864-LacZ construct. This is high copy (pUC), AmpR

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Type

Empty backbone

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pZS1katGp-RBS33-TP901-proD-oxyR

Plasmid

#78219

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCas2B

Plasmid

#82373

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Promoter

PEZ3

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCas8

Plasmid

#82380

Purpose

dCas9 without a promoter in acsA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Promoter

none

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas7

Plasmid

#82379

Purpose

dCas9 expressed from low constituve promoter, c225, in acsA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Promoter

c225

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas2E

Plasmid

#82376

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCG

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Promoter

PEZ3

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas2A

Plasmid

#82372

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Promoter

PEZ3

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas56

Plasmid

#82394

Purpose

sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas55

Plasmid

#82393

Purpose

sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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MT79

Plasmid

#63112

Purpose

The artificial microRNA is designed for down-regulating expression of ACD6 mRNA.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

amiR-ACD6

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pSA54

Plasmid

#35113

Depositor

John McCusker

Article

Ito-Harashima et al Yeast. 2004 Jan 15;21(1):53-61.

Insert

callys5MX3

Use

Yeast replicating

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pSA50

Plasmid

#35111

Depositor

John McCusker

Article

Ito-Harashima et al Yeast. 2004 Jan 15;21(1):53-61.

Insert

calys5MX4

Use

Yeast replicating

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2-F3-GCT

Plasmid

#30741

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F3 GCT

Use

Mrna production

Tags

FLAG tag

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pRF TpC RE7

Plasmid

#171046

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE7

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE7

Use

Luciferase

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1:ChrH

Plasmid

#257760

Purpose

For expression of untagged ChrH

Depositor

Wilfred van der Donk

Article

Chen et al Proc Natl Acad Sci U S A. 2026 Apr 7;123

Insert

ChrH

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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