NAX_CAGGS_CAS9_iRFP713
(Plasmid
#167860)
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PurposePiggyBac compatible plasmid expressing spCas9
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167860 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepB-CAGGS
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Vector typeCRISPR
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Selectable markersiRFP713
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NAX_CAGGS_CAS9_iRFP713 was a gift from Alejandro Chavez & Nan Cher Yeo (Addgene plasmid # 167860 ; http://n2t.net/addgene:167860 ; RRID:Addgene_167860) -
For your References section:
Generation and application of a versatile CRISPR toolkit for mammalian cell engineering. Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo. Synthetic Biology, Volume 6, Issue 1, 2021 10.1093/synbio/ysab033
