Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,715 results for: ski

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AAV-Nppa-Cre

Plasmid

#247326

Purpose

Expresses Cre recombinase under the control of the Nppa Promoter.

Depositor

William Pu, Mason Sweat

Article

Sweat et al Nat Cardiovasc Res. 2023 Oct;2(10):881-8

Insert

Nppa-Cre

Use

AAV

Expression

Mammalian

Mutation

TagRFP is out of frame and therefore not translat…

Promoter

Nppa Proximal promoter

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy FAM136A-HA mutant

Plasmid

#244882

Purpose

In vitro transcription of mutant human FAM136A downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A mutant (FAM136A Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Mutation

GRCh38, chr2:70300842G>A

Promoter

SP6

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy FAM136A-HA WT

Plasmid

#244883

Purpose

In vitro transcription of wild-type FAM136A downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A (FAM136A Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy ENDOG-HA

Plasmid

#244881

Purpose

In vitro transcription of human ENDOG downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

ENDOG (ENDOG Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-FAM136A-mutant-3xFLAG-hygro

Plasmid

#244879

Purpose

Overexpression of mutant human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A mutant (FAM136A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

GRCh38, chr2:70300842G>A

Promoter

EF1a

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 puro HTRA2 sg2

Plasmid

#244864

Purpose

Knockout of human HTRA2

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

HTRA2 sgRNA (HTRA2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 puro HTRA2 sg1

Plasmid

#244863

Purpose

Knockout of human HTRA2

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

HTRA2 sgRNA (HTRA2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR IMMP2L sg2

Plasmid

#244852

Purpose

Knockout of human IMMP2L

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

IMMP2L sgRNA (IMMP2L Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR IMMP2L sg1

Plasmid

#244851

Purpose

Knockout of human IMMP2L

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

IMMP2L sgRNA (IMMP2L Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-9c)

Plasmid

#241053

Purpose

Expresses SARS-CoV-2 spike (B.1-9c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-9c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,R158A,T478K,E484P,F490R,S494R,K529L,D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-9a)

Plasmid

#241051

Purpose

Expresses SARS-CoV-2 spike (B.1-9a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-9a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,Y248D,R403T,N439R,T478K,E484V,F490R,S494R,D6…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-9b)

Plasmid

#241052

Purpose

Expresses SARS-CoV-2 spike (B.1-9b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-9b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,S155R,T478K,E484V,F490R,S494R,K529L,D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8c)

Plasmid

#241049

Purpose

Expresses SARS-CoV-2 spike (B.1-8c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,S155R,N439R,T478K,E484V,K529L,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8d)

Plasmid

#241050

Purpose

Expresses SARS-CoV-2 spike (B.1-8d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,Y248D,R403T,N439R,T478K,E484V,F490R,S494R,D6…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8a)

Plasmid

#241047

Purpose

Expresses SARS-CoV-2 spike (B.1-8a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,R158A,K147N,N439R,T478K,S494R,F490R,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8b)

Plasmid

#241048

Purpose

Expresses SARS-CoV-2 spike (B.1-8b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S155R,R403T,N439R,T478K,E484V,K529L,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-5a)

Plasmid

#241046

Purpose

Expresses SARS-CoV-2 spike (B.1-5a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-5a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76I,M153S,Y473F,N481T,Q493R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2

Plasmid

#218557

Purpose

Encodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-HSF1-VIRF2

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC4 (pP18(T)_C11-AVA4065)

Plasmid

#239302

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC4

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC4 (EXOSC4 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-deltaPEST

Plasmid

#221118

Purpose

FAP tagged STE3-PEST mutant (delta413-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

MFA-IgKappa-FAPoptim-STE3-deltaPEST

Expression

Yeast

Mutation

STE3 mutant that lacks the PEST domain in the C-t…

Promoter

STE3

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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