We narrowed to 8,684 results for: App

1. pDEST40-CES2A-ΔC-S227A

   Plasmid

   #200556

   Purpose

   Mammalian expression of secreted mouse CES2A S227A mutant

   Depositor

   Jonathan Long

   Article

   Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

   Insert

   Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
   

   Use

   Tags

   6xHis, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Tbg-CES2A-ΔC

   Plasmid

   #200558

   Purpose

   Secreted mouse CES2A driven by heptaocyte-specific promoter in AAV viral vector

   Depositor

   Jonathan Long

   Article

   Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

   Insert

   Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mutation

   Promoter

   TBG promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGS-CD64-2

   Plasmid

   #109191

   Purpose

   Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   Full-length engineered CD64, derived from human CD64
   

   Use

   Tags

   Twin-Strep

   Expression

   Insect

   Mutation

   engineered for efficient expression, see publicat…

   Promoter

   Polyhedrin

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a_ASCL3_P2A_Hygro_Barcode

   Plasmid

   #120428

   Purpose

   Barcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   ASCL3 (ASCL3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-EGFP-C1-HsNot8-D40AE42A_AH

   Plasmid

   #148902

   Purpose

   Mammalian Expression of HsNot8-D40AE42A

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

   Insert

   HsNot8-D40AE42A (CNOT8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmiRGLO-miR-124-sponge_rev(neg. CTRL)

   Plasmid

   #117326

   Purpose

   Dual luciferase assay negative control for miR-124 binding

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   reverse miR-124 sponge
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAR-9.47_VP2_AS

   Plasmid

   #74241

   Purpose

   Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.

   Depositor

   Miguel Sena-Esteves

   Article

   Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

   Inserts

   AAV2 Rep
   AAV9.47-VP2_AS
   

   Use

   AAV

   Tags

   19 alanines fused to VP2 of AAV9.47

   Expression

   Mutation

   Promoter

   Available since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1a_HOXA11_P2A_Hygro_Barcode

   Plasmid

   #120453

   Purpose

   Barcoded lentiviral vector to express HOXA11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   HOXA11 (HOXA11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. rgef-1p::NmBirA

   Plasmid

   #79971

   Purpose

   encodes E.coli biotin-ligase under neuronal promotor for C.elegans

   Depositor

   Baris Tursun

   Article

   Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

   Insert

   BirA
   

   Use

   Tags

   NLS::myc

   Expression

   Worm

   Mutation

   Promoter

   rgef

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLCHKO_GFP_Luciferase

    Plasmid

    #155079

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a_KLF11_P2A_Hygro_Barcode

    Plasmid

    #120497

    Purpose

    Barcoded lentiviral vector to express KLF11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF11 (KLF11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. aL-L3

    Plasmid

    #103881

    Purpose

    full-length human aL with moxsynGFP in betapropeller

    Depositor

    Timothy Springer

    Article

    Nordenfelt et al Nat Commun. 2017 Dec 11;8(1):2047. doi:

    Insert

    full-length human aL with moxsynGFP in betapropeller (ITGAL Human)
    

    Use

    Tags

    tension sensor module

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-NoTag

    Plasmid

    #105767

    Purpose

    Control vector for pKK series (encodes only SLIC arms (TEV-L and TEV-R)); expression without tag. Useful to design other vectors; any tag can be added.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLJM1 Flag GFP 4E-BP1 Rheb15

    Plasmid

    #112761

    Purpose

    express GFP-4EBP1-Rheb15

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag eGFP and Rheb15

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPI

    Plasmid

    #184335

    Purpose

    Expression of GPI-anchored form of mouse Nrx3-alpha containing the alternative splice site 5 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,3,

    Depositor

    Peter Scheiffele

    Article

    Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

    Insert

    Nrxn3-alpha-GPI (Nrxn3 Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWZLblasti-HA-MEK1 C121S

    Plasmid

    #53165

    Purpose

    Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C121S

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pKK-FLAG-TEV

    Plasmid

    #105768

    Purpose

    Expression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pyr pUAST-HA

    Plasmid

    #69768

    Purpose

    Pyramus (FGF8-like-2) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

    Depositor

    Arno Muller

    Article

    Klingseisen et al Development. 2009 Jul;136(14):2393-402.

    Insert

    Pyramus (FGF8like-2) (pyr Fly)
    

    Use

    P element-based puast vector for gal4-regulated e…

    Tags

    HA-Tag

    Expression

    Insect

    Mutation

    241T amino acid insertion compared to reference s…

    Promoter

    hsp70 promoter

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pGEX-6P-1 AUP1 292-410

    Plasmid

    #185331

    Purpose

    Bacterial expression of AUP1 with GST fusion protein for binding assays

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 292-410 only

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-7 asyn S87E

    Plasmid

    #36059

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

    Insert

    alpha-synuclein S87E (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S87E

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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