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Showing: 6401 - 6420 of 40299 results

  8. pTS2326

    Plasmid
    #169628
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    Sept. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    169628-plasmid-map-sequence-id-372400

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  9. pTS2321

    Plasmid
    #169626
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    Sept. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    169626-plasmid-map-sequence-id-372399

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  10. pTS2322

    Plasmid
    #169627
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and Tactb-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169627-plasmid-map-sequence-id-365626

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  11. pTS2312

    Plasmid
    #169622
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169622-plasmid-map-sequence-id-365612

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  12. pTS2317

    Plasmid
    #169624
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169624-plasmid-map-sequence-id-365608

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  13. pJET-col2a1a-KI-donor

    Plasmid
    #184876
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka col2a1a locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Christoph Winkler
    Article
    Hui et al Development. 2022 May 20. pii: 275483. d
    Inserts
    Collagen2a1a 5' homology arm
    Collagen2a1a 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    EGFP
    Use
    CRISPR and Cre/Lox
    Available Since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    184876-plasmid-map-sequence-id-362292

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  14. S+Q111R

    Plasmid
    #167173
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+Q111R
    ATP1B1
    Expression
    Bacterial and Insect
    Mutation
    Changed Q at 111 to R
    Promoter
    P10 and PH
    Available Since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167173-plasmid-map-sequence-id-334482

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  15. S+N122D

    Plasmid
    #167174
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with N122D mutation in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+N122D
    ATP1B1
    Expression
    Bacterial and Insect
    Mutation
    Changed N at 111 to D
    Promoter
    P10 and PH
    Available Since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167174-plasmid-map-sequence-id-334489

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  17. pGG431

    Plasmid
    #165615
    Purpose
    Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165615-plasmid-map-sequence-id-330960

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Showing: 6401 - 6420 of 40299 results