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FLAG-GRP78-NLS-Mut

Plasmid

#235206

Purpose

Expresses GRP78 with mutated NLS in mammalian cells

Depositor

Amy Shiu Lee

Article

Liu et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Mutate the NLS sequence

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAK208_2xBRD9BD-IFP2.0C

Plasmid

#233734

Purpose

Expresses BRD9 Bromodomain as tandem double domain fused to C-terminal part of IFP2.0 for histone acetylation detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Genes (Basel). 2025 Apr 10;16(4):444. do

Insert

BRD9 (BRD9 Human)

Tags

IFP2.0 C-terminal part

Expression

Mammalian

Mutation

Bromodomain cloned twice in tandem

Promoter

CMV

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLIC-His6-MBP-TEV-Rgs14

Plasmid

#236395

Purpose

Expresses His6-MBP-TEV-Rgs14 in E. coli

Depositor

John Hepler

Article

Brown et al J Biol Chem. 2015 Apr 3;290(14):9037-49.

Insert

Rattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)

Tags

hexahistidine (H6) tag and maltose-binding protei…

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pOET3-6xHis-hSMSr

Plasmid

#236224

Purpose

Express N-terminal His-tagged human SMSr (SAMD8) protein in insect cells

Depositor

Chiaki Murakami

Article

Murakami et al J Biol Chem. 2021 Jan-Jun;296:100454. do

Insert

SAMD8 (SAMD8 Human)

Tags

Hexa histidine tag (6xHis-tag) and enterokinase s…

Expression

Insect

Mutation

wild type

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-TDP-43 (K408R)

Plasmid

#235493

Purpose

Expression of human TDP-43-EGFP with K408R mutation to block SUMOylation

Depositor

Maxime Rousseaux

Article

Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

Insert

TARDBP (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

K408R

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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PX4

Plasmid

#223340

Purpose

Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

ABCD1-EGFP-miniTurboID-3HA (PXA1 Mustard Weed)

Tags

EGFP-miniTurboID-3HA

Expression

Plant

Promoter

CaMV35S

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PX3

Plasmid

#223339

Purpose

Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

ABCD1-miniTurboID-EGFP-3HA (PXA1 Mustard Weed)

Tags

miniTurboID-EGFP-3HA

Expression

Plant

Promoter

CaMV35S

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PX2

Plasmid

#223338

Purpose

Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

ABCD1-EGFP-TurboID-3HA (PXA1 Mustard Weed)

Tags

EGFP-TurboID-3HA

Expression

Plant

Promoter

CaMV35S

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PX1

Plasmid

#223337

Purpose

Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

ABCD1-TurboID-EGFP-3HA (PXA1 Mustard Weed)

Tags

TurboID-EGFP-3HA

Expression

Plant

Promoter

CaMV35S

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MT2

Plasmid

#223330

Purpose

Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

3HA-TurboID-EGFP-OMP25 (Synj2bp Rat)

Tags

3HA-TurboID-EGFP

Expression

Plant

Promoter

CaMV35S

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MT3

Plasmid

#223331

Purpose

Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

3HA-EGFP-miniTurboID-OMP25 (Synj2bp Rat)

Tags

3HA-EGFP-miniTurboID

Expression

Plant

Promoter

CaMV35S

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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MT4

Plasmid

#223332

Purpose

Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

3HA-miniTurboID-EGFP-OMP25 (Synj2bp Rat)

Tags

3HA-miniTurboID-EGFP

Expression

Plant

Promoter

CaMV35S

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper DGK zeta human

Plasmid

#224575

Purpose

To downregulate the expression of human DGK zeta in human cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

Insert

sh RNAi Diacylglycerol kinase zeta human (DGKZ Human)

Use

RNAi

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper Retro GFP DGK alfa mouse

Plasmid

#224576

Purpose

Negative Control for downregulation of the expression of human DGK alfa in human cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

Insert

sh RNAi Diacylglycerol kinase alfa mouse (Dgka Mouse)

Use

RNAi and Retroviral

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper Retro GFP DGK zeta human

Plasmid

#224579

Purpose

To stably downregulate the expression of human DGK zeta in human cells

Depositor

Isabel Mérida

Article

Torres-Ayuso et al Oncogenesis. 2015 Aug 24;4(8):e164. doi:

Insert

sh RNAi Diacylglycerol kinase zeta human (GTPBP6 Human)

Use

RNAi and Retroviral

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN

Plasmid

#209786

Purpose

Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

TNT4, TadA8e, SpRY N, inteinN

Use

AAV

Promoter

TNT4

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-V5-APEX2 (in pLEX_307)

Plasmid

#214921

Purpose

constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

V5-APEX2

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

Plasmid

#207136

Purpose

DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)

Expression

Mammalian

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-Nedd1-mOrange2

Plasmid

#196860

Purpose

Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mOrange2 (Nedd1 Rat)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182-V5His6_H

Plasmid

#146499

Purpose

Insect Expression of DmGW182

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmGW182 (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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