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pHAGE-BRAF-T599I

Plasmid

#116201

Purpose

Lentiviral expression of BRAF T599I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

T599I

Promoter

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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B52 + FANCM sgSTOP

Plasmid

#100710

Purpose

B52 plasmid expressing FANCM sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting FANCM (cloned using BbsI) (FANCM Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoters

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-V600_K601delinsE

Plasmid

#116203

Purpose

Lentiviral expression of BRAF V600_K601delinsE

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

V600_K601delinsE

Promoter

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-EGR3-P2A-mCherry

Plasmid

#203861

Purpose

Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

EGR3 (EGR3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Kir6.2 K+ channel scFv [N363/71]

Plasmid

#190546

Purpose

Mammalian Expression of Kir6.2 K+ channel scFV. Derived from hybridoma N363/71.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir6.2 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj11 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Calbindin scFv [L109/57]

Plasmid

#182071

Purpose

Mammalian Expression of Calbindin scFv. Derived from hybridoma L109/57.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Calbindin (Homo sapiens) (CALB1 Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-E110delE

Plasmid

#116469

Purpose

Lentiviral expression of PIK3CA E110delE

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Tags

Expression

Mutation

E110delE

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-DDX3X-E285K

Plasmid

#116226

Purpose

Lentiviral expression of DDX3X E285K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DDX3X (DDX3X Human)

Use

Lentiviral

Tags

Expression

Mutation

E285K

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-L747_P753delinsS

Plasmid

#116268

Purpose

Lentiviral expression of EGFR L747_P753delinsS

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Expression

Mutation

L747_P753delinsS

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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NGFR 210Y177

Plasmid

#27487

Depositor

Warren Pear

Article

He et al Blood. 2002 Apr 15;99(8):2957-68.

Insert

bcr/abl (P210) (ABL1, BCR Human)

Use

Retroviral

Tags

IRES and NGFR

Expression

Mammalian

Mutation

Tyrosine 177 mutated to Phenylalanine (Y177F)

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only
pHAGE-BRAF-V600M

Plasmid

#116208

Purpose

Lentiviral expression of BRAF V600M

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

V600M

Promoter

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-T599_V600insP

Plasmid

#116199

Purpose

Lentiviral expression of BRAF T599_V600insP

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

T599_V600insP

Promoter

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-AXL-C311F

Plasmid

#116116

Purpose

Lentiviral expression of AXL C311F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AXL (AXL Human)

Use

Lentiviral

Tags

Expression

Mutation

C311F

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-N345K

Plasmid

#116517

Purpose

Lentiviral expression of PIK3CA N345K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Tags

Expression

Mutation

N345K

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G466E

Plasmid

#116150

Purpose

Lentiviral expression of BRAF G466E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

G466E

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-R93P

Plasmid

#116539

Purpose

Lentiviral expression of PIK3CA R93P

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Tags

Expression

Mutation

R93P

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR3-D786N

Plasmid

#116382

Purpose

Lentiviral expression of FGFR3 D786N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR3 (FGFR3 Human)

Use

Lentiviral

Tags

Expression

Mutation

D786N

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-AKT1-R121W

Plasmid

#116104

Purpose

Lentiviral expression of AKT1 R121W

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT1 (AKT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

R121W

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-M1043I

Plasmid

#116510

Purpose

Lentiviral expression of PIK3CA M1043I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Tags

Expression

Mutation

M1043I

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pQhsCDtRNA+

Plasmid

#86014

Purpose

Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene

Depositor

Hong Li

Article

Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

Inserts

U14-tRNA chimera (SNORD14A Human)
15.5K (SNU13 Human)
fibrillarin (FBL Human)
NOP56 (NOP56 Human)
NOP58 (NOP58 Human)

Use

Co-expression; purification

Tags

6x-His

Expression

Bacterial

Mutation

amino acids 1-401, amino acids 1-411, and amino a…

Promoter

LPP and Ptac

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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