We narrowed to 13,040 results for: Mpl

1. pCu-Atg17(416) (I325D)

   Plasmid

   #121407

   Purpose

   Expressing Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-ATG17 ORF-CYC1 terminator
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Isoleucine 325 to Aspartic acid

   Promoter

   CUP1 promoter

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBMN mCherry-NBR1(F562A/Y731A/ΔUBD)

   Plasmid

   #119692

   Purpose

   For mammalian expression of LIR double mutant NBR1 without its UBD

   Depositor

   Michael Lazarou

   Article

   Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

   Insert

   NBR1 (NBR1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   F562A/Y731A & UBD deletion (1-906 ), See depo…

   Promoter

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBMN iRFP670-OPTN(F178A/D474N)

   Plasmid

   #119694

   Purpose

   For mammalian expression of iRFP-tagged LIR/UBD double mutant OPTN

   Depositor

   Michael Lazarou

   Article

   Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

   Insert

   OPTN (OPTN Human)
   

   Use

   Retroviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   F178A/D474N

   Promoter

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACEBAC1-CoREST-C

   Plasmid

   #109159

   Purpose

   Encodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Inui et al Protein Sci. 2017 May;26(5):997-1011. do

   Insert

   human C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Jan. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL15

   Plasmid

   #117430

   Purpose

   Expression of ARL2

   Depositor

   Richard Kahn

   Article

   Clark et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

   Insert

   ARL2 (ARL2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)

   Plasmid

   #115432

   Purpose

   Vector for expressing RPL28 with mutagenized exon2

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   exon 2 deleted, with MfeI / NheI cloning site

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFL I3

   Plasmid

   #113514

   Purpose

   Expression of I3 in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   I3 (PPP1R11 Human)
   

   Use

   Tags

   6*His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19_4xT790_28bp

   Plasmid

   #114720

   Purpose

   4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standard

   Depositor

   Nils Walter

   Article

   Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19_T790_28bp

   Plasmid

   #114722

   Purpose

   28 bp fragment of the WT EGFR allele used as SiMREPS analyte standard

   Depositor

   Nils Walter

   Article

   Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIG1_UL136_19kDa_EE

    Plasmid

    #74951

    Purpose

    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Insert

    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    

    Use

    Lentiviral

    Tags

    Glu-Glu tag (EYMPME)

    Expression

    Mammalian

    Mutation

    delta 1-127

    Promoter

    CMV, lentiviral LTR

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIG1_UL136_23kDa_Myc

    Plasmid

    #74949

    Purpose

    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Insert

    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    

    Use

    Lentiviral

    Tags

    HA Tag (YPYDVPDYA)

    Expression

    Mammalian

    Mutation

    delta 1-99 M128A

    Promoter

    CMV, Lentiviral LTR

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3- myc NES-mCE(K294A) NLS

    Plasmid

    #82467

    Purpose

    Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NLS deleted from mouse Capping Enzyme (Rngtt Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of KRKY from 573-657

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3-biotin-myc- mCE

    Plasmid

    #82475

    Purpose

    Expresses mRNA capping enzyme containing N terminal biotin and myc tag

    Depositor

    Daniel Schoenberg

    Article

    Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

    Insert

    Biotin (Rngtt Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a_6HT-TBP6.7(WT)

    Plasmid

    #112720

    Purpose

    Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(WT)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a_6HT-TBP6.7(P46A)

    Plasmid

    #112721

    Purpose

    This mutation (P46A) marks the first residue within the evolved region of U1A.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P46A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P46A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a_6HT-TBP6.7(Q48T)

    Plasmid

    #112725

    Purpose

    This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48T)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48T

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a_6HT-TBP6.7(P51A)

    Plasmid

    #112728

    Purpose

    Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P51A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P51A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a_6HT-TBP6.7(Q54A)

    Plasmid

    #112730

    Purpose

    Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q54A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q54A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-Flag-Sesn-C mSesn2 (delAB)

    Plasmid

    #111799

    Purpose

    Mammalian Expression of mSesn2

    Depositor

    Jun Hee Lee

    Article

    Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

    Insert

    mSesn2 (Sesn2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AB domain truncation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-Flag-Sesn-B+C mSesn2 (delA)

    Plasmid

    #111798

    Purpose

    Mammalian Expression of mSesn2

    Depositor

    Jun Hee Lee

    Article

    Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

    Insert

    mSesn2 (Sesn2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    A domain truncation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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