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pCS2-FLAG-Rab11

Plasmid

#194336

Purpose

Expresses FLAG tagged Rab11 in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Publication

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Rab11 (RAB11A Rabbit)

Tags

FLAG

Expression

Mammalian

Promoter

CMV IE94

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-Rab8

Plasmid

#194335

Purpose

Expresses FLAG tagged Rab8 in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Publication

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Rab8 (RAB8A Canis lupus)

Tags

FLAG

Expression

Mammalian

Promoter

CMV IE94

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF3A DD 590-702

Plasmid

#193927

Purpose

Mammalian expression of GFP-tagged Kif 3A DD 590-702

Depositor

Marvin Bentley

Publication

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-KIF3A DD 590-702

Plasmid

#193928

Purpose

Mammalian expression of Halo-tagged Kif 3A DD 590-702

Depositor

Marvin Bentley

Publication

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Tags

Halo

Expression

Mammalian

Promoter

Chicken Beta actin

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF3A CC 419-589

Plasmid

#193925

Purpose

Mammalian expression of GFP-tagged Kif 3A CC 419-589

Depositor

Marvin Bentley

Publication

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE3_D125I_47-300_mCh-SspB (pBS1071)

Plasmid

#185294

Purpose

For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_D125I_47-300

Expression

Mammalian

Mutation

D125I

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-hFAM3B-FLAG

Plasmid

#180146

Purpose

Coding sequence of human FAM3B in pCS2 with C terminal FLAG tag

Depositor

Edward De Robertis

Publication

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

FAM3B (FAM3B Human)

Tags

FLAG

Expression

Mammalian

Promoter

SP6

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-dEGF-exon1

Plasmid

#177261

Purpose

A knockout vector for the dog Egf

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-dEGF-5’UTR

Plasmid

#177260

Purpose

A knockout vector for the dog Egf

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-dNrg1

Plasmid

#173696

Purpose

A knockout vector for the dog Nrg1.

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Nrg1 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dEreg

Plasmid

#173697

Purpose

A knockout vector for the dog Ereg.

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dTgfa

Plasmid

#173698

Purpose

A knockout vector for the dog Tgfa.

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dHbegf

Plasmid

#173699

Purpose

A knockout vector for the dog Hbegf.

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dEgf

Plasmid

#173700

Purpose

A knock-out vector for the dog Egf

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_R1368W-talin1 (1-2541)

Plasmid

#166117

Purpose

For expression of full-length mouse talin-1 carrying mutation R1368W in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Publication

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

R1368W-talin1-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

R1368W

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFA-DAB2

Plasmid

#162690

Purpose

Expresses DabAB2 inorganic carbon transport complex

Depositor

David Savage

Publication

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB2 inorganic carbon transport complex

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_EIF2S3_GTP-EFTU

Plasmid

#109829

Purpose

Protein expression and purification of EIF2S3_GTP-EFTU

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EIF2S3_GTP-EFTU (EIF2S3 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140169

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thaliana

Depositor

Benjamin Glick

Publication

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-DsRed-Express2-FKBP(LV)(C22V)

Expression

Plant

Promoter

CaMV 35S

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTEI127

Plasmid

#126067

Purpose

Module 1 vector of PlaMiNGo toolkit for expression of mitochondria targeted GFP1-10 in plant cell

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-HSPE-sh1

Plasmid

#92033

Purpose

pSIREN-RetroQ vector containing shRNA sequence to HPSE

Depositor

Joe Landry

Publication

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #1 to HPSE (Hpse Mouse)

Use

Retroviral

Available since

July 19, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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