We narrowed to 10,016 results for: ADA

1. HA-AR-LBD

   Plasmid

   #171239

   Purpose

   Mammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   AR truncate

   Promoter

   CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_NESPylRS(AF)_hU6tRNAPyl

   Plasmid

   #223508

   Purpose

   PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

   Inserts

   NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y306A; Y384F

   Promoter

   CVM, SP6 and U6

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C1-AR Q0

   Plasmid

   #86427

   Purpose

   Fluorescent human androgen receptor (fused to EGFP) with deleted polyglutamine region

   Depositor

   Michael Mancini

   Article

   Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

   Insert

   androgen receptor (AR) (AR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of the poly-glutamine expansion

   Promoter

   CMV

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

   Plasmid

   #190790

   Purpose

   [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

   Depositor

   Paul Hudson

   Article

   Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

   Inserts

   Cas9
   mazF-LVA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   LVA

   Expression

   Bacterial

   Mutation

   silent mutations of BpiI-sites in original Cas9 g…

   Promoter

   PnrsB and Ptrc-[E*](104)

   Available Since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. hSyn-DIO-somBiPOLES-mCerulean

   Plasmid

   #154951

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Has Service

   AAV Retrograde, AAV5, and AAV9

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEMHE-membrane-mEGFP

   Plasmid

   #105526

   Purpose

   T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   GNAI2 (GNAI2 Human)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Expression

   Mutation

   fragment encoding the plasma membrane targeting s…

   Promoter

   T7

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26

   Plasmid

   #92143

   Purpose

   Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   osTir1
   

   Use

   Mouse Targeting

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-IRES-Blast POLE1

   Plasmid

   #160802

   Purpose

   Express POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLE1 (POLE Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   shPOLE_1 and shPOLE2 resistant

   Promoter

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-moxGFP-Puro-LAMP1

   Plasmid

   #207788

   Purpose

   Donor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. CaMKII-somBiPOLES-mCerulean

    Plasmid

    #154948

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Has Service

    AAV Retrograde, AAV5, and AAV9

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa(0.4)

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSD025A

    Plasmid

    #218128

    Purpose

    Hybrid circuit displaying hsCRBN-W384A,Y386A bait (P22 anchor) and driving expression of gIII-neg

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    CRBN (CRBN Human)
    M13 pIII-neg
    

    Use

    Tags

    scP22

    Expression

    Mutation

    W384A, Y386A

    Promoter

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTwist-SARS-CoV-2 Spike-deltaC18 LB.1

    Plasmid

    #223277

    Purpose

    Encodes SARS-CoV-2 spike LB.1 for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike LB.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N1_MeCP2(deltaNIC)

    Plasmid

    #110191

    Purpose

    Expression of mouse MeCP2 (deltaNIC) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta N and C termini. Intervening region replace…

    Promoter

    CMV

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-C-mStayGold-Puro-CANX

    Plasmid

    #227281

    Purpose

    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CANX locus. For cilia visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CANX Homology Arms flanking a mStayGold-2A-Puro Cassette (CANX Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 Mfn2-HA S27A

    Plasmid

    #139193

    Purpose

    Expresses C-terminal HA tagged human Mfn2 S27A in mammalian cells

    Depositor

    Allan Weissman

    Article

    Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

    Insert

    MFN2 (MFN2 Human)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    Serine 27 to Alanine

    Promoter

    CMV promoter

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 Mfn2-HA S27D

    Plasmid

    #139194

    Purpose

    Expresses C-terminal HA tagged human Mfn2 S27D in mammalian cells

    Depositor

    Allan Weissman

    Article

    Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

    Insert

    MFN2 (MFN2 Human)
    

    Use

    Tags

    2HA

    Expression

    Mammalian

    Mutation

    Serine 27 to Aspartic Acid

    Promoter

    CMV promoter

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCrisprV2 sgRNA hCRY1 c-term

    Plasmid

    #179453

    Purpose

    Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    sgRNA targeting human CRY1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-DIO-GRAB_CRF1.0

    Plasmid

    #208661

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSH-EFIRES-P-Seipin-miniIAA7-mScarlet-I

    Plasmid

    #129721

    Purpose

    AAVS1 safe harbor targeting vector expressing miniIAA7-mScarlet-I tagged Seipin

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    Seipin (BSCL2 Human)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    miniIAA7-mScarlet-I

    Expression

    Mammalian

    Mutation

    silent mutations

    Promoter

    EF1a

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. GPS-MYC

    Plasmid

    #160130

    Purpose

    A reporter that monitors MYC protein expression.

    Depositor

    Channing Der

    Article

    Blake et al Sci Signal. 2019 Jul 16;12(590). pii: 12

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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