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pCJH026_proD_Cas12c-multiplexing_CAM

Plasmid

#183073

Purpose

Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJL32

Plasmid

#174066

Purpose

Encodes for CIB1 fused to VP16 in S. cerevisiae. Part of optogenetic system.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pGal1-LacO-3NLS-CIB1-VP16-tADH1

Tags

FLAG

Expression

Yeast

Promoter

pGal1

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup-4Ala-mut_C

Plasmid

#146031

Purpose

Insect Expression of DmCup-4Ala-mut

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup-4Ala-mut (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_1-417-V5His6_C

Plasmid

#146032

Purpose

Insect Expression of DmCup_1-417

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_1-417 (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_419-771-V5His6_C

Plasmid

#146033

Purpose

Insect Expression of DmCup_419-771

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_419-771 (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_1-417-Y342AL347A-V5His6_M

Plasmid

#146901

Purpose

Insect Expression of DmCup_1-417-Y342AL347A

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_1-417-Y342AL347A (cup Fly)

Expression

Insect

Available Since

Feb. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pJL45

Plasmid

#174067

Purpose

Expresses CIB1 in S. cerevisiae. Includes inducible lac operon.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pGal1-LacO-3NLS-CIB1-tADH1

Tags

FLAG

Expression

Yeast

Promoter

pGal1

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCk1chlo

Plasmid

#163942

Purpose

Acceptor plasmid for odd level position 1

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk2chlo

Plasmid

#163943

Purpose

Acceptor plasmid for odd level position 2

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk3chlo

Plasmid

#163944

Purpose

Acceptor plasmid for odd level position 3

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk4chlo

Plasmid

#163945

Purpose

Acceptor plasmid for odd level position 4

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsAchlo

Plasmid

#163946

Purpose

Acceptor plasmid for even level position 1

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsBchlo

Plasmid

#163947

Purpose

Acceptor plasmid for even level position 2

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsCchlo

Plasmid

#163948

Purpose

Acceptor plasmid for even level position 3

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsEchlo

Plasmid

#163949

Purpose

Acceptor plasmid for even level position 4

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PROM5_Ntprrn_rev_chlo

Plasmid

#163952

Purpose

L0 part - promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

from GenBank: KT364744.3

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PROM5_Ntpsba_chlo

Plasmid

#163953

Purpose

L0 part - promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

from GenBank: KT364744.3

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3TERM_Ntpsba_rev_chlo

Plasmid

#163932

Purpose

L0 part - terminator

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Nicotiana tabacum psba terminator

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGEM-Isce-Tol2-Tn5-neo

Plasmid

#176619

Purpose

for generating PCR templates with Tol2 repeats and Isce sites for BAC recombination

Depositor

Nathan Lawson

Article

Shih et al Development. 2021 Nov 9. pii: 273393. do

Insert

I-SceI sites, Tol2 repeats and Tn5-neo cassette

Use

Unspecified

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSBbi-TIR1-tTA/Zeo

Plasmid

#171685

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (zeocin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

Zeocin

Expression

Mammalian

Promoter

RPBSA promoter

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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