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pENTR223-BRLF1(Rta)

Plasmid

#195824

Purpose

Gateway Entry Clone of Epstein-Barr virus BRLF1

Depositor

Eric Johannsen

Publication

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BRLF1 (BRLF1 Epstein-Barr virus)

Use

Gateway Cloning

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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10279-M28-663

Plasmid

#225662

Purpose

Lentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholding

Depositor

Dominic Esposito

Publication

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCopGFP

Use

Lentiviral

Promoter

mCMVp

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-mCherry-Binder

Plasmid

#179139

Purpose

Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

Depositor

Klaus Hahn

Publication

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

SspB

Tags

FLAG and mCherry

Expression

Mammalian

Mutation

Y11K/A15E, residue numbering from pdb: 1ou9

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE DddA6-11 1397C

Plasmid

#221938

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6/11 1397C

Depositor

Stephen Ekker

Publication

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

TALE: Q231R, W232G, S233A | DddA: T1413I

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX DdCBE DddA6-11 1397C

Plasmid

#221933

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA6/11 1397C

Depositor

Stephen Ekker

Publication

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

DddA: T1413I

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-hGSDMC

Plasmid

#218937

Purpose

Expression of N-terminal FLAG tagged human gasdermin C

Depositor

Christopher LaRock

Publication

LaRock et al Nature. 2022 May;605(7910):527-531. doi:

Insert

human GSDMC (GSDMC Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pBa.MARK2-Halo

Plasmid

#224414

Purpose

Mammalian expression of MARK2

Depositor

Marvin Bentley

Publication

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MARK2 (MARK2 Human)

Tags

Halo

Expression

Mammalian

Promoter

Chicken Beta actin

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19/DdmRuby3

Plasmid

#154305

Purpose

cloned with the codon-optimized mRuby3 gene for expression in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Publication

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

mRuby3

Expression

Bacterial

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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MIP-T3

Plasmid

#99511

Purpose

to express MIP-T3 in mammalian system

Depositor

Dong-Yan Jin

Publication

Ng et al J Immunol. 2011 Dec 15;187(12):6473-82.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MIP-T3 (TRAF3IP1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(1004)

Plasmid

#44366

Depositor

Hal Alper

Publication

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(1004) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(1004)

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pET15b-At 14-3-3 lambda

Plasmid

#44942

Depositor

Robert Ferl

Publication

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 lambda (GRF6 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 nu

Plasmid

#44940

Depositor

Robert Ferl

Publication

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 nu (GRF7 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 mu

Plasmid

#44938

Depositor

Robert Ferl

Publication

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 mu (GRF9 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 phi

Plasmid

#44939

Depositor

Robert Ferl

Publication

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 phi (GF14 PHI Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
EF2451_pET21a(+)

Plasmid

#13840

Depositor

SeWon Suh

Publication

Kang et al Acta Crystallograph Sect F Struct Biol C

Insert

PPAT

Tags

His

Expression

Bacterial

Available since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-Lin28b-P3

Plasmid

#64794

Purpose

Luciferase expression driven by Lin28b promoter P3

Depositor

Joshua Mendell

Publication

Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lin28b promoter P3 (LIN28B Human)

Use

Luciferase

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PB CRIV Gc Spike H6

Plasmid

#240163

Purpose

Plasmid for making PiggyBac stable cell line expressing Cristoli virus (CRIV) glycoprotein Gc spike with a C-terminal His6 tag

Depositor

Stephen Graham

Publication

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

CRIV Gc spike

Use

Piggybac

Tags

6xHis

Expression

Mammalian

Mutation

contains residues 478-906 only

Available since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pOPTH OROV N

Plasmid

#229663

Purpose

Express Oropouche virus BeAn 19991 nucleoprotein with N-terminal His6 tag

Depositor

Stephen Graham

Publication

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

Nucleoprotein

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_hRNMT_RAM

Plasmid

#213528

Purpose

Expresses the human RNMT-RAM complex

Depositor

Thomas Tuschl

Publication

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Inserts

human RNMT (RNMT Human)
RAM

Tags

His6-sumo

Expression

Bacterial

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_ZIKV_NS5_MTase

Plasmid

#213529

Purpose

Expresses the MTase domain of NS5 of ZIKV

Depositor

Thomas Tuschl

Publication

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

ZIKA virus NS5 methyltransferase domain

Tags

His6-sumo

Expression

Bacterial

Mutation

MTase domain aa 1-265

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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