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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 77,445 results for: Mycs;

Showing: 6561 - 6580 of 77445 results

  1. L284F Cus2p

    Plasmid
    #73059
    Purpose
    S. cerevisiae Cus2p gene with L284F Mutation
    Depositor
    Aaron Hoskins
    Article
    Rodgers et al RNA. 2015 Dec 2.
    Insert
    CUS2p (CUS2 Budding Yeast)
    Use
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Mutation
    Leucine 284 to Phenylalanine
    Promoter
    T7
    Available since
    Availability
    Academic Institutions and Nonprofits only
    73059-plasmid-map-sequence-id-150795

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  2. D282N Cus2p

    Plasmid
    #73060
    Purpose
    S. cerevisiae Cus2p gene with D282N Mutation
    Depositor
    Aaron Hoskins
    Article
    Rodgers et al RNA. 2015 Dec 2.
    Insert
    CUS2p (CUS2 Budding Yeast)
    Use
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Mutation
    Asp 282 to Asn
    Promoter
    T7
    Available since
    Availability
    Academic Institutions and Nonprofits only
    73060-plasmid-map-sequence-id-150798

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  3. lentiCRISPR v2

    Plasmid
    #52961
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52961-plasmid-map-sequence-id-244694

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  5. pYPQAT57

    Plasmid
    #223429
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223429-plasmid-map-sequence-id-453502

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  8. pYC1640

    Plasmid
    #158719
    Purpose
    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    Sth1 sgRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158719-plasmid-map-sequence-id-320840

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  9. PB-TO-NFIA.1-SOX9 (bi-directional promoter)

    Plasmid
    #182306
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Inserts
    NFIA (NFIA Human)
    SOX9 (SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Piggybac
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-Bi-directional
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182306-plasmid-map-sequence-id-359516

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Showing: 6561 - 6580 of 77445 results