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pENTR223-CMV-Cre-U6-sgC0111-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209062

Purpose

Entry vector that encodes sgRNAs against mouse Rb1, Trp53, Rbl2, and a non-targeting sgRNA (C0111) and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

Insert

non-targeting sgRNA (C0111)

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet_Seq1.3

Plasmid

#206137

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1 (Col1a1 Mouse)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57-Cx26G45E-IRES-GCaMP6s

Plasmid

#188237

Purpose

3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 variant p.G45E and GCaMP6s

Depositor

Fabio Mammano

Article

Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

Insert

GJB2 (GJB2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

p.G45E

Promoter

Tight TRE promoter

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-R/G-ICUE

Plasmid

#181849

Purpose

ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

R/G-ICUE (RAPGEF3 Human)

Tags

mRuby2 and sfGFP

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG membrane-bound lipin-1 (delta200)

Plasmid

#120278

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Tags

FLAG and in-frame fusion with the delta200 Golgi-…

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_PSG2_WT

Plasmid

#81917

Purpose

Gateway Donor vector containing PSG2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PSG2 (PSG2 Human)

Use

Gateway entry vector

Mutation

V20L; p.333_335del-NPT-ins-IQH

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMR3A_WT

Plasmid

#81887

Purpose

Gateway Donor vector containing SMR3A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMR3A (SMR3A Human)

Use

Gateway entry vector

Mutation

C42R; P132L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_C1orf150_WT

Plasmid

#81842

Purpose

Gateway Donor vector containing C1ORF150 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C1ORF150 (GCSAML Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPAG8_WT

Plasmid

#81848

Purpose

Gateway Donor vector containing SPAG8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPAG8 (SPAG8 Human)

Use

Gateway entry vector

Mutation

E2D; E5G; P226L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CPXCR1_WT

Plasmid

#81819

Purpose

Gateway Donor vector containing CPXCR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CPXCR1 (CPXCR1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_OR2M2_WT

Plasmid

#81758

Purpose

Gateway Donor vector containing OR2M2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

R220G, C235R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MTM1_p.R253Q

Plasmid

#81724

Purpose

Gateway Donor vector containing MTM1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MTM1 (MTM1 Human)

Use

Gateway entry vector

Mutation

R253Q; E410K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MTM1_p.P226S

Plasmid

#81678

Purpose

Gateway Donor vector containing MTM1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MTM1 (MTM1 Human)

Use

Gateway entry vector

Mutation

P226S; E410K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_OR2M2_p.N42Y

Plasmid

#81638

Purpose

Gateway Donor vector containing OR2M2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

N42Y; R220G; C235R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_OR2M2_p.V117I

Plasmid

#81639

Purpose

Gateway Donor vector containing OR2M2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

V117I; R220G; C235R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK19_p.E88K

Plasmid

#81649

Purpose

Gateway Donor vector containing STK19, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STK19 (STK19 Human)

Use

Gateway entry vector

Mutation

E88K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TPK1_WT

Plasmid

#82926

Purpose

Gateway Donor vector containing TPK1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

TPK1 (TPK1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM34_WT

Plasmid

#82929

Purpose

Gateway Donor vector containing RBM34, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM34 (RBM34 Human)

Use

Gateway entry vector

Mutation

deleted amino acids 1-5

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_OR2M2_p.E232V

Plasmid

#81634

Purpose

Gateway Donor vector containing OR2M2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

R220G; E232V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_OR2M2_p.P182S

Plasmid

#81635

Purpose

Gateway Donor vector containing OR2M2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

P182S; R220G; C235R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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