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pAG415Gal_Gcn4 9acidA

Plasmid

#232967

Purpose

Galactose iduced expression of Gcn4 9acidA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

TEV cleavage site

Expression

Yeast

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 E2K2

Plasmid

#232968

Purpose

Galactose iduced expression of Gcn4 EK+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 EK+

Tags

TEV cleavage site

Expression

Yeast

Mutation

S104K, S117E, S136E, S144K

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 9acidAGFP

Plasmid

#233009

Purpose

Galactose iduced expression of Gcn4 9acidAGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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ppAG415Gal_Gcn4 L113A+

Plasmid

#232972

Purpose

Galactose iduced expression of Gcn4 L113A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L113A

Tags

TEV cleavage site

Expression

Yeast

Mutation

L113A, E114Y, N126H, D127E, S136A

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoED

Plasmid

#232957

Purpose

Galactose iduced expression of Gcn4 ILVtoED in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123D, I128E, V130E, V135E, L137D, I142D

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Swap3HA

Plasmid

#232998

Purpose

Galactose iduced expression of Gcn4 k0.47HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.47

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 StoL

Plasmid

#232981

Purpose

Galactose iduced expression of Gcn4 StoL in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 StoL

Tags

TEV cleavage site

Expression

Yeast

Mutation

S104L, S117L, S136L, S144L

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WW+

Plasmid

#232984

Purpose

Galactose iduced expression of Gcn4 WW+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 F108A+

Plasmid

#232974

Purpose

Galactose iduced expression of Gcn4 F108A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F108A

Tags

TEV cleavage site

Expression

Yeast

Mutation

F108A, T121L, A141P

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_DBDGFP

Plasmid

#232999

Purpose

Galactose iduced expression of Gcn4 DBDGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

DBD

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoAS

Plasmid

#232959

Purpose

Galactose iduced expression of Gcn4 ILVtoAS in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoAS

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAM239 Rpn10

Plasmid

#226357

Purpose

Expression of yeast Rpn10

Depositor

Andreas Martin

Publication

Beckwith et al Nat Struct Mol Biol. 2013 Oct;20(10):116

Insert

rpn10 (RPN10 Budding Yeast)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAM315 pACYC-His6-Rpn10[ΔUIM]

Plasmid

#226348

Purpose

Expression of yeast Rpn10 with the UIM deleted

Depositor

Andreas Martin

Publication

Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

Insert

rpn10 (RPN10 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

delta UIM

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAM263

Plasmid

#226907

Purpose

expression of yeast Ubc1

Depositor

Andreas Martin

Publication

Beckwith et al Nat Struct Mol Biol. 2013 Oct;20(10):116

Insert

ubc1 (UBC1 Budding Yeast)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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LDB34

Plasmid

#185431

Purpose

Yeast expression of Nup1 C-terminal truncation (nup1-21 allele)

Depositor

Ken Belanger, Laura Davis

Publication

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation at amino acid 1041 from the C-term of …

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB33

Plasmid

#185430

Purpose

Yeast expression of Nup1 N-terminal truncation (nup1-15 allele)

Depositor

Ken Belanger, Laura Davis

Publication

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation of amino acids 3-191 from the N-term o…

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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p{3P3-mTurquoise::FRT,attB}

Plasmid

#182180

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

mTurquoise

Expression

Insect

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-DsRed::FRT,attB}

Plasmid

#182190

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-DsRed::FRT-RC,attB}

Plasmid

#182187

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{3P3-dsRed::KD,attB}

Plasmid

#182178

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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