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Showing: 641 - 660 of 848 results

  1. pDRF'- AmTryoshka1;3-LS-F138I-T78H

    Plasmid
    #100963
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
    Use
    Tags
    Expression
    Yeast
    Mutation
    F138I, T78H
    Promoter
    PMA
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100963-plasmid-map-sequence-id-193742

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  2. pET24a-VHH-tev-std

    Plasmid
    #109423
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109423-plasmid-map-sequence-id-260701

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  5. pLVTHM

    Plasmid
    #12247
    Purpose
    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid
    Depositor
    Didier Trono
    Article
    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12247-plasmid-map-sequence-id-352425

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  12. pOCC65

    Plasmid
    #118865
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, mGFP
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118865-plasmid-map-sequence-id-232317

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  13. pOCC61

    Plasmid
    #118877
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    mGFP, HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118877-plasmid-map-sequence-id-232346

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  16. pOCC139

    Plasmid
    #118872
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease and mGFP
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118872-plasmid-map-sequence-id-232236

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  18. pYFP-NEAT1pr_v1-RT

    Plasmid
    #97087
    Purpose
    Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v1 vector, but also works with px330-NEAT1pr_v2.
    Depositor
    Archa Fox
    Insert
    nuclear paraspeckle assembly transcript 1 (NEAT1 Human)
    Use
    CRISPR
    Tags
    EYFP
    Expression
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97087-plasmid-map-sequence-id-189642

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  19. pDsRed-TSTEP_v2

    Plasmid
    #72336
    Purpose
    Tissue-specifically swappable (C-terminal TagRFPT-to-EGFP) genome tagging plasmid with dsRed selection marker for injection mediated gene targeting (Drosophila melanogaster)
    Depositor
    Avital Rodal
    Article
    Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.
    Insert
    RRS-TagRFPT-p10-[3xP3dsRed]-RRS-EGFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    72336-plasmid-map-sequence-id-132003

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Showing: 641 - 660 of 848 results