Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 2,418 results for: EFS

Clear keywords
Showing: 1281 - 1320 of 2418 results

  1. LSB-hsa-miR-551b-3p

    Plasmid
    #103642
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-551b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-551b-3p target (MIR551B Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103642-plasmid-map-sequence-id-352625

    Enlarge

  2. LSB-hsa-miR-6511a-3p

    Plasmid
    #103705
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-6511a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-6511a-3p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103705-plasmid-map-sequence-id-196458

    Enlarge

  3. LSB-hsa-miR-6511a-5p

    Plasmid
    #103706
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-6511a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-6511a-5p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103706-plasmid-map-sequence-id-196460

    Enlarge

  4. lentiCRISPR - Amplicon, JAK2 sgRNA 2

    Plasmid
    #70660
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. LSB-hsa-miR-181d-5p

    Plasmid
    #103281
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-181d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-181d-5p target (MIR181D Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103281-plasmid-map-sequence-id-195611

    Enlarge

  6. LSB-hsa-miR-99a-3p

    Plasmid
    #103764
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-99a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-99a-3p target (MIR99A Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103764-plasmid-map-sequence-id-196576

    Enlarge

  7. LSB-hsa-miR-181b-5p

    Plasmid
    #103278
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-181b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-181b-5p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103278-plasmid-map-sequence-id-195605

    Enlarge

  8. LSB-hsa-miR-149-3p

    Plasmid
    #103254
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-149-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-149-3p target (MIR149 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103254-plasmid-map-sequence-id-195557

    Enlarge

  9. LSB-hsa-miR-149-5p

    Plasmid
    #103255
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-149-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 FPs. . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-149-5p target (MIR149 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103255-plasmid-map-sequence-id-195559

    Enlarge

  12. LSB-hsa-miR-302b-5p

    Plasmid
    #103402
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-302b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-302b-5p target (MIR302B Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103402-plasmid-map-sequence-id-195853

    Enlarge

  13. LSB-hsa-miR-148b-3p

    Plasmid
    #103252
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-148b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-148b-3p target (MIR148B Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103252-plasmid-map-sequence-id-195553

    Enlarge

  14. LSB-hsa-miR-484

    Plasmid
    #103552
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-484 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-484 target (MIR484 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103552-plasmid-map-sequence-id-196152

    Enlarge

  15. LSB-hsa-miR-630

    Plasmid
    #103690
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-630 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-630 target (MIR630 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103690-plasmid-map-sequence-id-196428

    Enlarge

  16. LSB-hsa-miR-633

    Plasmid
    #103691
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-633 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-633 target (MIR633 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103691-plasmid-map-sequence-id-196430

    Enlarge

  17. LSB-hsa-miR-135b-5p

    Plasmid
    #103229
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-135b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-135b-5p target (MIR135B Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103229-plasmid-map-sequence-id-195507

    Enlarge

  19. pPKm-235

    Plasmid
    #90509
    Purpose
    pSIN - EF-1alpha - MTS sHO1 - P2A - MTS - Hy2, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and phytochromobilin:ferredoxin oxidoreductase (Hy2)
    Depositor
    Todd Coleman
    Article
    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717
    Insert
    mito-sHO1, mito-Hy2
    Use
    Lentiviral
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    90509-plasmid-map-sequence-id-204693

    Enlarge

  22. pPB9v2

    Plasmid
    #210029
    Purpose
    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Article
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    Cas9
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210029-plasmid-map-sequence-id-414001

    Enlarge

  25. LVDP 2P.2.EGFP

    Plasmid
    #231905
    Purpose
    For the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging EGFP mRNA into the VLPs
    Depositor
    Sriram Neelamegham
    Article
    Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10
    Insert
    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, E, M SARS-CoV-2)
    Use
    Lentiviral
    Mutation
    R203M in N protein
    Promoter
    CMV, EF-1a
    Available Since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231905-plasmid-map-sequence-id-459042

    Enlarge

  28. pN1mcherry-NKD2EFMUT

    Plasmid
    #174333
    Purpose
    encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.
    Depositor
    Sonal Srikanth
    Article
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Tags
    mCherry tag
    Expression
    Mammalian
    Mutation
    NKD2132DFD134>AFA (substitutions of D132 and D…
    Promoter
    CMV
    Available Since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174333-plasmid-map-sequence-id-349872

    Enlarge

  31. LSB-hsa-miR-99b-3p

    Plasmid
    #103766
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-99b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-99b-3p target (MIR99B Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103766-plasmid-map-sequence-id-196580

    Enlarge

  32. LSB-hsa-miR-9-3p

    Plasmid
    #103748
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-9-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-9-3p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103748-plasmid-map-sequence-id-196544

    Enlarge

  33. LSB-hsa-miR-92a-3p

    Plasmid
    #103752
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-92a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-92a-3p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103752-plasmid-map-sequence-id-196552

    Enlarge

  34. LSB-hsa-miR-765

    Plasmid
    #103730
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-765 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-765 target (MIR765 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103730-plasmid-map-sequence-id-196508

    Enlarge

  35. LSB-hsa-miR-802

    Plasmid
    #103737
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-802 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-802 target (MIR802 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103737-plasmid-map-sequence-id-196522

    Enlarge

  36. LSB-hsa-miR-657

    Plasmid
    #103712
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-657 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-657 target (MIR657 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103712-plasmid-map-sequence-id-196472

    Enlarge

  37. LSB-hsa-miR-634

    Plasmid
    #103692
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-634 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-634 target (MIR634 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103692-plasmid-map-sequence-id-196432

    Enlarge

  38. LSB-hsa-miR-635

    Plasmid
    #103693
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-635 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-635 target (MIR635 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103693-plasmid-map-sequence-id-196434

    Enlarge

  39. LSB-hsa-miR-640

    Plasmid
    #103695
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-640 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-640 target (MIR640 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103695-plasmid-map-sequence-id-196438

    Enlarge

  40. LSB-hsa-miR-643

    Plasmid
    #103698
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-643 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-643 target (MIR643 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103698-plasmid-map-sequence-id-196444

    Enlarge

Showing: 1281 - 1320 of 2418 results