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Showing: 1601 - 1650 of 18154 results

  1. pJK549

    Plasmid
    #72520
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, large subunit
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72520-plasmid-map-sequence-id-132604

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  2. pJK555

    Plasmid
    #72521
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72521-plasmid-map-sequence-id-132606

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  3. pJK577

    Plasmid
    #72528
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, small subunit
    Use
    Gene synthesis
    Promoter
    n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72528-plasmid-map-sequence-id-132616

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  4. mGeos2-VESS-Lifeact-7

    Plasmid
    #54526
    Purpose
    Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581
    Depositor
    Michael Davidson
    Insert
    Lifeact-mGeos2-VESS
    Expression
    Mammalian
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. mKOet-Peroxisomes-2

    Plasmid
    #54534
    Purpose
    Localization: Peroxisomes, Excitation: 548, Emission: 561
    Depositor
    Michael Davidson
    Insert
    mKO-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…
    Expression
    Mammalian
    Available Since
    July 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. mGeos2-VEMS-Lifeact-7

    Plasmid
    #54818
    Purpose
    Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581
    Depositor
    Michael Davidson
    Insert
    Lifeact-mGeos2-VEMS
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pET15-MHL

    Plasmid
    #26092
    Purpose
    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    Sept. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    26092-plasmid-map-sequence-id-466943

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  8. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

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  9. pAG GST Empty

    Plasmid
    #236735
    Purpose
    Empty bacterial expression plasmid for GST fusion proteins
    Depositor
    Melina Fan, Addgene Research Program
    Type
    Empty backbone
    Tags
    GST
    Expression
    Bacterial
    Available Since
    April 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236735-plasmid-map-sequence-id-469201

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  11. sfGFP-Peroxisomes-2

    Plasmid
    #54601
    Purpose
    Localization: Peroxisomes, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    sfGFP-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54601-plasmid-map-sequence-id-267741

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  13. mCerulean-Peroxisomes-2

    Plasmid
    #54691
    Purpose
    Localization: Peroxisomes, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    mCerulean-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…
    Expression
    Mammalian
    Available Since
    July 25, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. mCitrine-Peroxisomes-2

    Plasmid
    #54672
    Purpose
    Localization: Peroxisomes, Excitation: 516, Emission: 529
    Depositor
    Michael Davidson
    Insert
    mCitrine-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pJK633

    Plasmid
    #72397
    Purpose
    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole synthetase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72397-plasmid-map-sequence-id-134629

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  16. mWasabi-Peroxisomes-2

    Plasmid
    #54639
    Purpose
    Localization: Peroxisomes, Excitation: 493, Emission: 509
    Depositor
    Michael Davidson
    Insert
    mWasabi-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. tdEos-Peroxisomes-2

    Plasmid
    #54544
    Purpose
    Localization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    tdEos-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1) = Ser-Lys-L…
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. mGeos2-VEL-Lifeact-7

    Plasmid
    #54817
    Purpose
    Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581
    Depositor
    Michael Davidson
    Insert
    Lifeact-mGeos2-VEL
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. pJK532

    Plasmid
    #72513
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit with N-terminal His6 tag (H6AaPurQ)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72513-plasmid-map-sequence-id-132539

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  20. pJK544

    Plasmid
    #72517
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, small subunit
    Expression
    Bacterial
    Mutation
    contains additional Ala2
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72517-plasmid-map-sequence-id-132599

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  30. pAIP4_5GUQ

    Plasmid
    #234171
    Purpose
    Heterologous protein expression of ASCH domain-containing ribonuclease in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5GUQ
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234171-plasmid-map-sequence-id-464137

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  31. pAIP4_1AKO

    Plasmid
    #234162
    Purpose
    Heterologous protein expression of exodeoxyribonuclease III (Exonuclease III) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1AKO (xthA Escherichia coli K-12)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234162-plasmid-map-sequence-id-464128

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  32. pLSU1/t35s[TMV]:MCS+:NosT

    Plasmid
    #212160
    Purpose
    This binary vector has an empty backbone with extended multiple cloning site (MSC) where you can inserta a gene into a cassette with a strong promoter (truncated 35sCAMV) and UTR (Tomato Mosaic Virus)
    Depositor
    Wayne Curtis
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212160-plasmid-map-sequence-id-422551

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  33. HA Display Part 1-4 DO

    Plasmid
    #206514
    Purpose
    CcdB Dropout vector for parts 1-4 of Hemagglutinin surface expression in mammalian cells
    Depositor
    IIya Finkelstein
    Type
    Empty backbone
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    206514-plasmid-map-sequence-id-424195

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  34. pJK323

    Plasmid
    #73495
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide synthetase
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73495-plasmid-map-sequence-id-135755

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  35. pJK322

    Plasmid
    #73494
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide mutase
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Promoter
    n/a
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73494-plasmid-map-sequence-id-135746

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  36. pJK614

    Plasmid
    #72387
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 4 silent mutations in codons 9-11 and 42…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72387-plasmid-map-sequence-id-133927

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  37. pJK621

    Plasmid
    #72388
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72388-plasmid-map-sequence-id-133928

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  38. pJK592

    Plasmid
    #72386
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurD)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72386-plasmid-map-sequence-id-133922

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  39. pJK684

    Plasmid
    #71638
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val mutant (TdPurE2-S38V)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to valine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71638-plasmid-map-sequence-id-131658

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  40. pJK685

    Plasmid
    #71639
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asn mutant (TdPurE2-S38N)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to asparagine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71639-plasmid-map-sequence-id-131659

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  41. pJK687

    Plasmid
    #71640
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met mutant (TdPurE2-K41M)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes lysine-41 to methionine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71640-plasmid-map-sequence-id-131661

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  42. pJK688

    Plasmid
    #71641
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Arg mutant (TdPurE2-K41R)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes lysine-41 to arginine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71641-plasmid-map-sequence-id-131662

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  43. pJK695

    Plasmid
    #71644
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp mutant (TdPurE2-H17W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes histidine-17 to tryptophan
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71644-plasmid-map-sequence-id-131665

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  44. pJK696

    Plasmid
    #71645
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met15>Trp mutant (TdPurE2-M15W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes methionine-15 to tryptophan
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71645-plasmid-map-sequence-id-131666

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  45. pJK607

    Plasmid
    #71626
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Cys mutant (TdPurE2-S38C)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to cysteine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71626-plasmid-map-sequence-id-131645

    Enlarge

  46. pJK617

    Plasmid
    #71627
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Ala mutant (TdPurE2-S38A)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to alanine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71627-plasmid-map-sequence-id-131646

    Enlarge

  47. pJK643

    Plasmid
    #71628
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr mutant (TdPurE2-S38T)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to threonine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71628-plasmid-map-sequence-id-131647

    Enlarge

  48. pJK644

    Plasmid
    #71629
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp mutant (TdPurE2-S38D)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to aspartate
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71629-plasmid-map-sequence-id-131648

    Enlarge

  49. pJK553

    Plasmid
    #72452
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72452-plasmid-map-sequence-id-133889

    Enlarge

  50. pJK533

    Plasmid
    #72514
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, small subunit
    Expression
    Bacterial
    Mutation
    contains additional Ala2
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72514-plasmid-map-sequence-id-135820

    Enlarge

Showing: 1601 - 1650 of 18154 results