We narrowed to 9,400 results for: Coli

1. pInt_attP1_tsXis_sacB_kanR

   Plasmid

   #205302

   Purpose

   Integrating plasmid for ORBIT in E. coli with self excising factor (Xis) and sacB for counter selection. Used for markerless modifications.

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK615

   Plasmid

   #71699

   Purpose

   Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK651

   Plasmid

   #71702

   Purpose

   Produces E. coli thioredoxin reductase with a C-terminal His6 tag (EcTrxBH6)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin reductase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ec dnaX(T-only)-pET(3c)

   Plasmid

   #237237

   Purpose

   over-express E. coli PolX (Pol III tau subunit - no gamma)

   Depositor

   Michael O'Donnell

   Insert

   dnaX (tau only) (dnaX E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ec holB-pET(11a)(ACH-)

   Plasmid

   #237234

   Purpose

   over-express E. coli holB (Pol III delta prime subunit)

   Depositor

   Michael O'Donnell

   Insert

   holB (delta prime) (holB E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAIP4_1JL1

   Plasmid

   #234206

   Purpose

   Heterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1JL1 (rnhA Escherichia coli)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAIP4_1AKO

   Plasmid

   #234162

   Purpose

   Heterologous protein expression of exodeoxyribonuclease III (Exonuclease III) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1AKO (xthA Escherichia coli K-12)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCASCADE-gltA2-udhA

   Plasmid

   #65819

   Purpose

   gltA-udhA targeting guide RNA array E. coli , Low Phosphate Induction

   Depositor

   Michael Lynch

   Insert

   gltA2-udhA guide array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induc…

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDC10

   Plasmid

   #75077

   Purpose

   Produces Escherichia coli glycinamide ribonucleotide synthetase (H6EcPurD)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Insert

   phosphoribosylglycinamide synthetase (purD Escherichia coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   adds unique SacII site at Thr95-Ala96 by a silent…

   Promoter

   T7

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMVA2RBS035

    Plasmid

    #121051

    Purpose

    Expresses recombinant MVA pathway in Escherichia coli

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    mvaE
    mvaS
    mvaK1
    mvaK2
    mvaD
    idi
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBB75-ncRNA

    Plasmid

    #194087

    Purpose

    Expresses Ec86 ncRNA in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 ncRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21b-rrt-His8

    Plasmid

    #194088

    Purpose

    Expresses Ec86 reverse transcriptase in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 rrt
    

    Use

    Tags

    His8

    Expression

    Mutation

    Promoter

    T7

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCapV

    Plasmid

    #183028

    Purpose

    the patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28

    Depositor

    Ute Romling

    Article

    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(

    Insert

    patatin-like phospholipase CapV
    

    Use

    Tags

    No

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD promoter

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEcRNAP5

    Plasmid

    #128939

    Purpose

    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z

    Depositor

    Seth Darst

    Article

    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

    Inserts

    RpoAppx234-241-(His)10 (rpoA Escherichia coli)
    RpoB
    RpoC
    RpoZ
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Residues 234-241 are substituted for a PreScissio…

    Promoter

    T7

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACYC-GroEL/ES-TF

    Plasmid

    #83923

    Purpose

    Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Gro EL/ES
    Trigger Factor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAIDA1

    Plasmid

    #79180

    Purpose

    A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

    Depositor

    Gen Larsson

    Article

    Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

    Insert

    Adhesin Involved in Diffuse Adherence
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFV2-HA

    Plasmid

    #187082

    Purpose

    Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)

    Depositor

    Steven Vik

    Article

    Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1

    Insert

    atpBEFHAGDC
    

    Use

    Tags

    6-His beta subunit (atpD) and HA tag subunit a (a…

    Expression

    Bacterial

    Mutation

    All Cys codons changed to Ala.

    Promoter

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJS119

    Plasmid

    #207592

    Purpose

    GFP-TonB deltaII expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-TonB deltaII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJS104

    Plasmid

    #207589

    Purpose

    GFP-TolA deltaII expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-TolA deltaII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJS70

    Plasmid

    #207586

    Purpose

    GFP-TolA H22A expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-TolA H22A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H22A

    Promoter

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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