164,802 results

1. pCBC-MT3T4

   Plasmid

   #50595

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T3, gRNA scaffold, U6-26t plus, OsU3p, T4
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. KIF1A(1-393)-GCN4-2xmCherry-EF(C)

   Plasmid

   #61665

   Purpose

   Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   KIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6-5'+3' MS2-CircRNA

   Plasmid

   #213752

   Purpose

   For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-CMV GFP-LC3B WT

   Plasmid

   #123114

   Purpose

   Expresses EGFP-LC3B wild-type in mammalian cells. Fluorescent reporter for autophagosomes.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSH-EFIRES-P-AtAFB2-mCherry

   Plasmid

   #129716

   Purpose

   AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF1a

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a_MYC_P2A_Hygro_Barcode

   Plasmid

   #120461

   Purpose

   Barcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-SET-FLAG-HA

   Plasmid

   #24998

   Depositor

   Judy Lieberman

   Article

   Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

   Insert

   SET (SET Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. tau/pET29b

   Plasmid

   #16316

   Depositor

   Peter Klein

   Article

   Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

   Insert

   tau (MAPT Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)

   Plasmid

   #140892

   Purpose

   Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.

   Depositor

   Yasushi Okamura

   Article

   Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

   Insert

   CiDr-VSP L223F-mCherry (tpte, vsp Ciona intestinalis, Zebrafish)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   changed 223L to F in Dr-VSP (zebrafish TPTE)

   Promoter

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. ancBE4-SpRY

    Plasmid

    #226855

    Purpose

    Expresses ancBE4max-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ancBE4max-SpRY
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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