162,221 results

1. Sender_plasmid2

   Plasmid

   #140693

   Purpose

   Encodes inducible expression of RhlI (C4-HSL quorum sensing synthase)

   Depositor

   Matthew Bennett

   Article

   Alnahhas et al ACS Synth Biol. 2019 Sep 20;8(9):2051-20

   Insert

   RhlI (rhlI )
   

   Use

   Tags

   ssrA degradation tag (ASV)

   Expression

   Bacterial

   Mutation

   Promoter

   engineered lac promoter (IPTG inducible)

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRISPR_HLA_class_I_1

   Plasmid

   #164986

   Purpose

   Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   gRNA against HLA class I
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFD4-U6:1_U6:3tandemgRNAs

   Plasmid

   #49411

   Purpose

   expression of two gRNAs from Drosophila U6:1 and U6:3

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Inserts

   dU6-1:gRNA
   dU6-3:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-1 and dU6-3

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHIV-EGFP

   Plasmid

   #21373

   Purpose

   self-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFP

   Depositor

   Bryan Welm, Zena Werb

   Article

   Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-tTRKRAB-red

   Plasmid

   #12250

   Depositor

   Didier Trono

   Article

   Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

   Insert

   TetR-KRAB fusion and dsRED
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   dsRed added to backbone.

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-NLS-AcrIIA4

   Plasmid

   #113037

   Purpose

   Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SV40 NLS-AcrIIA4
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

   Plasmid

   #90086

   Purpose

   This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA

   Depositor

   Raffaella Sordella

   Article

   Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Destabilized Domain and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor

   Plasmid

   #229787

   Purpose

   Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   LacZ; ZF10-NS3-p65
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. TUBB5-Halo

   Plasmid

   #64691

   Purpose

   Expresses beta-tubulin labeled with Halo-tag

   Depositor

   Yasushi Okada

   Article

   Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

   Insert

   TUBB
   

   Use

   Tags

   Halo-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti PGK Puro DEST (w529-2)

    Plasmid

    #19068

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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