We narrowed to 7,729 results for: Pol

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pYihI-N-term S-A

Plasmid

#233096

Purpose

Expression of GST-YihI with N-term serines (S) mutated to alanine (A)

Depositor

Michael Downey

Publication

Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1

Insert

YihI with N-term serines (S) mutated to alanine (A)

Tags

GST

Expression

Bacterial

Mutation

N-term serines (S) mutated to alanine (A)

Available since

May 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NSP12:4393-5324_Nbio_Chis_BV

Plasmid

#234377

Purpose

Baculovirus expression for structure determination. May not contain entire coding region of gene.

Depositor

Cheryl Arrowsmith

Insert

Nsp12_SARS2

Use

Baculovirus expresssion

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Promoter

Polyhedrin

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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NSP13:5325-5925_BVnAvi

Plasmid

#234375

Purpose

Baculovirus expression for structure determination. May not contain entire coding region of gene.

Depositor

Cheryl Arrowsmith

Insert

Nsp13_SARS2

Use

Baculovirus expresssion

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Promoter

Polyhedrin

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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SARS1_NSP13_BV_NBIO-TEV_CTHROM-his

Plasmid

#234382

Purpose

Baculovirus expression for structure determination. May not contain entire coding region of gene.

Depositor

Cheryl Arrowsmith

Insert

Nsp13_SARS

Use

Baculovirus expresssion

Tags

LVPRGSGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Promoter

Polyhedrin

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN10-Pct3-(–)-Eldh1(cHis6)-noAccs(ElA2)

Plasmid

#233880

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Publication

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh1 (Elen_0511 Eggerthella lenta)

Tags

His

Expression

Bacterial

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN8-Pct3-(+)-Eddh(cHis6)-noAccs(ElA2)

Plasmid

#233879

Purpose

Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Publication

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eddh (Elen_0501 Eggerthella lenta)

Tags

His

Expression

Bacterial

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF12-Pct3-(–)-Eldh1(cHis6)-Accs(ElA2)

Plasmid

#233870

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Publication

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh1 (Elen_0511 Eggerthella lenta)

Tags

His

Expression

Bacterial

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF10-Pct3-(+)-Eddh(cHis6)-Accs(ElA2)

Plasmid

#233869

Purpose

Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Publication

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eddh (Elen_0501 Eggerthella lenta)

Tags

His

Expression

Bacterial

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCARGO-RMCE-Airn

Plasmid

#233266

Purpose

CARGO plasmid containing ~90kb dox-inducible Airn transgene

Depositor

Mauro Calabrese

Publication

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Insert

Airn (Airn Mouse)

Expression

Mammalian

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN6-Elen0497-ElVadh-noAccs(ElDSM2243)

Plasmid

#225314

Purpose

Expresses Eggerthella lenta DSM 2243 El Vadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Publication

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

El Vadh (Elen_0497 Eggerthella lenta)

Expression

Bacterial

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF8-HcdhA(cHis8)BC-HcdR-Accs(ElA2)

Plasmid

#225303

Purpose

Expresses Eggerthella lenta A2 Hcdh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Publication

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Hcdh

Tags

His8

Expression

Bacterial

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.CBX8ΔChromo

Plasmid

#224708

Purpose

Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

Depositor

Chen Davidovich

Publication

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 chromodomain truncation (CBX8 Human)

Tags

HMBP

Expression

Insect

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.CBX8

Plasmid

#224707

Purpose

Expresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Publication

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 (CBX8 Human)

Tags

HMBP

Expression

Insect

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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NylB-SCY_pET29b

Plasmid

#215415

Purpose

Expression construct for NylB-SCY gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Publication

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB-SCY

Expression

Bacterial

Mutation

R187S/F264C/D370Y

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFBMBD1a-C-GFP (pc1564)

Plasmid

#211711

Purpose

Expresses fl. MBD1 GFP tagged in insect cells

Depositor

Cristina Cardoso

Publication

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD1

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-NUbo-E3(48)-VSV

Plasmid

#212741

Purpose

Consitutive expression of NUbo-E3(48)-VSV in budding yeast

Depositor

Helle Ulrich

Publication

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pRSITEP-shBIRC6-2(3)-C911

Plasmid

#202452

Purpose

a seed-matched control for pRSITEP-puro-shBIRC6-2(3)

Depositor

William Hahn

Publication

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRSITEP-shBIRC6-2-C911

Plasmid

#202450

Purpose

a seed-matched control for pRSITEP-puro-shBIRC6-2

Depositor

William Hahn

Publication

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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TBPL1 KO 1

Plasmid

#200210

Purpose

gRNA for TBPL1 KO

Depositor

Sheila Teves

Publication

Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

Insert

TBP1 KO 1 (Tbpl1 Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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TBPL1 KO 2

Plasmid

#200211

Purpose

gRNA for TBPL1 KO

Depositor

Sheila Teves

Publication

Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

Insert

TBP1 KO 2 (Tbpl1 Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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