We narrowed to 9,174 results for: Pol

1. pMAPLe4 PCMTD1

   Plasmid

   #246503

   Purpose

   Recombinant protein expression of PCMTD1

   Depositor

   Steven Clarke

   Article

   Warmack et al Biochemistry. 2022 May 17;61(10):879-894

   Insert

   Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Residue 312 is Isoleucine in this plasmid but occ…

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1A-DDHD2-30-202-Hygro

   Plasmid

   #238101

   Purpose

   Expression of structurally predicted WWE domain of DDHD2 with EGFP on C terminal

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   DDHD2 (DDHD2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 30-202 only

   Promoter

   EF1alpha

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-EF1A-TRIP12-391-938-EGFP-Hygro

   Plasmid

   #238092

   Purpose

   Expression of structurally predicted WWE domain of TRIP12 with EGFP on C terminal

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   TRIP12 (TRIP12 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 319-938 only

   Promoter

   EF1alpha

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1A-HUWE1-1503-1780_EGFP-Hygro

   Plasmid

   #238096

   Purpose

   Expression of structurally predicted WWE domain of HUWE1 with EGFP on C terminal

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-166 only

   Promoter

   EF1alpha

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-Hygro-EF1A-Af1521(K35E/Y145R)-myc

   Plasmid

   #196237

   Purpose

   Af1521(K35E/Y145R) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   Af1521(K35E/Y145R) encoding a C-terminal myc tag
   

   Use

   Lentiviral

   Tags

   myc tag

   Expression

   Mammalian

   Mutation

   amino acid 35 lysine is replaced with glutamic ac…

   Promoter

   EF1A

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T-3-GST-Tev-RNF146(100-182)-myc

   Plasmid

   #196240

   Purpose

   N-terminal GST fusion of RNF146(100-182) WWE domain with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   N-terminal GST fusion of RNF146(100-182) with a TEV protease site between GST and RNF146(100-182) encoding a C-terminal myc tag
   

   Tags

   GST tag and myc tag

   Expression

   Bacterial

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNK091

   Plasmid

   #219769

   Purpose

   MoClo-compatible Level P vector carrying fungal bioluminescence system lacking luciferase gene

   Depositor

   Karen Sarkisyan

   Article

   Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

   Insert

   pNos-KanR-ocsT | p35S-nnHispS-ocsT | pCmYLCV-npgA-ATPT | p35S-nnCPH-ocsT | pFMV-nnH3H-nosT
   

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   pNos-KanR-ocsT | p35S-nnHispS-ocsT | pCmYLCV-npgA…

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. WT USP7 UBL-12

   Plasmid

   #227230

   Purpose

   Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Article

   Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   aa 535-775

   Promoter

   T7-promoter

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-WzxE-TEV-His10

   Plasmid

   #226781

   Purpose

   Expression of C-terminally TEV cleavable His10-tagged WzxE from E. coli in E. coli

   Depositor

   James Naismith

   Article

   Le Bas et al Open Biol. 2025 Jan;15(1):240310. doi: 1

   Insert

   wzxE
   

   Tags

   TEV cleavable 10xHistidine tag

   Expression

   Bacterial

   Mutation

   valine insertion in position 2 due to the cloning…

   Promoter

   araBAD

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTag4C_SP-DRD2-V2R-NTEV-tcs-GV-2xHA

    Plasmid

    #227103

    Purpose

    DRD2 receptor for split TEV assay

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    DRD2-NTEV (DRD2 Human)
    

    Tags

    2xHA

    Expression

    Mammalian

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG8Ha-His-EWSR1-359-549

    Plasmid

    #195868

    Purpose

    Expresses the RRM-RG2-ZnF (359-549) of EWS

    Depositor

    David Libich

    Article

    Selig et al Biopolymers. 2023 May;114(5):e23536. doi

    Insert

    EWS 359-549 (EWSR1 Human)
    

    Tags

    His8-linker-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNK7190

    Plasmid

    #219763

    Purpose

    MoClo-compatible Level M for expression nnLuz, nnH3H, nnCPH in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    nnLuz, nnH3H, nnCPH
    

    Expression

    Plant

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX459v3-eSpCas9(1.1)

    Plasmid

    #178800

    Purpose

    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX459v3-SpCas9-HF1

    Plasmid

    #178801

    Purpose

    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNK189

    Plasmid

    #219743

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiens

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal hispidin-3-hydroxylase
    

    Use

    Synthetic Biology

    Mutation

    D37E, V181I, S323M, M385K

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNK4169

    Plasmid

    #219744

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastoris

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal hispidin-3-hydroxylase
    

    Use

    Synthetic Biology

    Mutation

    D37E, V181I, S323M, M385K

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNK7183

    Plasmid

    #219760

    Purpose

    MoClo-compatible Level 1 for expression PzPKS2 in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    PzPKS2
    

    Expression

    Plant

    Promoter

    p35s_0.4kb - 5'UTR TMV omega

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNK7180

    Plasmid

    #219762

    Purpose

    MoClo-compatible Level 1 for expression HmS in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    HmS
    

    Expression

    Plant

    Promoter

    p35s_0.4kb - 5'UTR TMV omega

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFB1.HMBP.PrS.EZH2-mt2

    Plasmid

    #220240

    Purpose

    Expression of an EZH2 mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2 (EZH2 Human)
    

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

    Promoter

    Polyhedrin

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFB1.HMBP.PrS.EZH2-mt1

    Plasmid

    #220239

    Purpose

    Expression of an EZH2 mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 1 (EZH2 Human)
    

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    R27A, R31A, F165A

    Promoter

    Polyhedrin

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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