We narrowed to 16,131 results for: form

1. II801: pMVP (L5-L4) ecDHFR DD

   Plasmid

   #121721

   Purpose

   pMVP L5-L4 entry plasmid, contains ecDHFR DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term ecDHFR degron domain (stabilized by TMP) to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   ecDHFR Degron Domain (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDF1-PTPRD-P1690F

   Plasmid

   #32525

   Depositor

   Todd Waldman

   Article

   Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

   Insert

   Protein tyrosine phosphatase receptor-type delta P1690F (PTPRD Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   P1690F

   Available Since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pTPTP alpha (S204A)

   Plasmid

   #17694

   Depositor

   Xinmin Zheng

   Article

   Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

   Insert

   PTPalpha S204A (PTPRA Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S204A

   Available Since

   April 24, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pCEP4-SERTG484E-myc

   Plasmid

   #107471

   Purpose

   Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G484E decreased transport and surface expression relative to wildtype

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Serotonin Transporter (SERT) (SLC6A4 Human)
   

   Expression

   Mammalian

   Mutation

   G484E

   Promoter

   CMV

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-SERTS477F-myc

   Plasmid

   #107470

   Purpose

   Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S477F loss of transport relative to wildtype, decreased surface expression

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Serotonin Transporter (SERT) (SLC6A4 Human)
   

   Expression

   Mammalian

   Mutation

   S477F

   Promoter

   CMV

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. KJ801: pMVP/Hygro-DEST

   Plasmid

   #121858

   Purpose

   pMVP destination vector, empty expression vector backbone w/ Hygro selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Pmvp gateway destination vector

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MolBC

   Plasmid

   #92210

   Purpose

   Expression of H. influenzae MolBC (ABC Transporter) in E. coli

   Depositor

   Douglas Rees

   Article

   Pinkett et al Science. 2007 Jan 19;315(5810):373-7. Ep

   Inserts

   MolC (HI1471 )
   MolB (HI1470 )
   

   Tags

   8x Histidine Tag and Enterokinase Cleavage Site b…

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSUPER-ARF3b

   Plasmid

   #67496

   Purpose

   Expression of shRNA targeting human ARF3b

   Depositor

   Richard Kahn

   Article

   Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

   Insert

   HsARF3 shRNA (ARF3 Human)
   

   Use

   RNAi

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pSUPER-ARF4b

   Plasmid

   #67498

   Purpose

   Expression of shRNA targeting human ARF4b

   Depositor

   Richard Kahn

   Article

   Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

   Insert

   HsARF4 shRNA (ARF4 Human)
   

   Use

   RNAi

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pHcPro (GB0067)

    Plasmid

    #68196

    Purpose

    Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    TEV HcPro coding region
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. K347CLM G234A/43/347

    Plasmid

    #24454

    Depositor

    Ron Vale

    Article

    Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

    Insert

    Kinesin (KIF5B Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S, G234A/S43C/E3…

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

12. TOPO pGK

    Plasmid

    #68452

    Purpose

    TOPO construct expressing pGK for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Phosphoglycerate Kinase 1 Promoter (PGK1 Human)
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E-Dnm2b-DN

    Plasmid

    #109574

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative Dynamin2b

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Dynamin2b (dnm2b Zebrafish)
    

    Use

    Zebrafish plasmids

    Mutation

    dominant negative

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3E-EHD1b-dEH

    Plasmid

    #109567

    Purpose

    Multisite gateway vector for 3' tagging with EHD1b-delta-EH

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    EHD1b (ehd1b Zebrafish)
    

    Use

    Zebrafish plasmids

    Mutation

    delta-EH

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. LB801: pMVP/PB/eCFP-DEST

    Plasmid

    #121872

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ eCFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. B. thetaiotaomicron mRNA toehold switch sensor

    Plasmid

    #110707

    Purpose

    Toehold switch sensor to detect a species specific mRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    B. thetaiotaomicron species specific toehold switch sensor
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-ratFDH

    Plasmid

    #135813

    Purpose

    Expresses rat FDH (also referred to as AldH1L1) in mammalian cells.

    Depositor

    Sergey Krupenko

    Article

    Krupenko et al Cell Growth Differ. 2002 May;13(5):227-3

    Insert

    Aldh1l1 (Aldh1l1 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFR092

    Plasmid

    #134148

    Purpose

    Expression of CENP-T

    Depositor

    Iain Cheeseman

    Article

    Nishino et al EMBO J. 2013 Feb 6;32(3):424-36. doi: 10

    Insert

    CENP-T (CENPT Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-375

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCEP4-SERTI425V-myc

    Plasmid

    #107464

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425V conferring aproximately wildtype phenotype

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Expression

    Mammalian

    Mutation

    I425V

    Promoter

    CMV

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. KU701: pMVP (L5-L4) eGFP

    Plasmid

    #121707

    Purpose

    pMVP L5-L4 entry plasmid, contains eGFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eGFP fusion to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eGFP (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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