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M6570

Plasmid

#219773

Purpose

MoClo-compatible Level 0 vector carrying promoter pORCA3

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

Promoter ORCA3

Use

Synthetic Biology

Expression

Plant

Promoter

pORCA3

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pMH-SpAsp1

Plasmid

#238315

Purpose

SpAsp1 protein expression in bacteria

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

asp1 (asp1 Fission Yeast)

Tags

His7-Tag, Strep-tag II, SUMO protein

Expression

Bacterial

Mutation

SpAsp1

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-mCherry

Plasmid

#216174

Purpose

Generate lentiviruses to act as a positive transduction control for pHAGE2-TetO vectors in pluripotency reprogramming

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

mCherry

Use

Lentiviral

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF5)-TS.FF5x1-bGH

Plasmid

#235277

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mGL-bGH

Plasmid

#235299

Purpose

mGreenLantern expression to serve as a transfection marker, EF1a-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF4)-TS.FF6x1-bGH

Plasmid

#235282

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF4

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF5)-TS.FF6x1-bGH

Plasmid

#235260

Purpose

ComMAND open-loop circuit regulating mRuby2 with miRE-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-mGL-bGH

Plasmid

#235255

Purpose

mGreenLantern expression to serve as a transfection marker, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-PTPN12-Clover

Plasmid

#215527

Purpose

Expression vector with clover-tagged PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Tags

Clover

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pINDUCER20-PTPN12

Plasmid

#215517

Purpose

Dox-inducible lentiviral transfer vector with PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Lentiviral

Promoter

mCMV, Doxycycline-inducible

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-PTPN12-Clover

Plasmid

#215521

Purpose

Gateway entry vector with clover-tagged PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Gateway entry vector

Tags

Clover

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-Src(E378G)

Plasmid

#215462

Purpose

Gateway entry vector with Src constitutively-active mutant (E378G)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Gateway entry vector

Mutation

E378G (constitutively-active)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-VPS35-mScarlet

Plasmid

#215472

Purpose

Gateway entry vector with VPS35 tagged with mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

VPS35 (VPS35 Human)

Use

Gateway entry vector

Tags

mScarlet

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-Src(Y527F)

Plasmid

#215460

Purpose

Gateway entry vector with Src constitutively-active mutant (Y527F)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Gateway entry vector

Mutation

Y527F (constitutively-active)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-Src(K295R)

Plasmid

#215461

Purpose

Gateway entry vector with Src kinase-dead mutant (K295R)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Gateway entry vector

Mutation

K295R (Kinase dead)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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hPGK-mRuby2-bGH

Plasmid

#235254

Purpose

ComMAND base gene regulating mRuby2, hPGK-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

hPGK (human phosphoglycerate kinase)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF5)-TS.FF6x1-bGH

Plasmid

#235276

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235270

Purpose

ComMAND open-loop circuit regulating mRuby2, EF1a

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235271

Purpose

ComMAND closed-loop circuit regulating mRuby2, EF1a

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2(miR.FF4)-TS.FF4x1-bGH

Plasmid

#235283

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF4

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge