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Plasmid#175802PurposePlasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tagExpressionBacterialMutationE184QPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAC690-TEF
Plasmid#160432PurposeExpresses eGFP under control of the yeast TEF2 promoterDepositorInsertS. cerevisiae TEF2 promoter
UseTagsExpressionYeastMutationPromoterS. cerevisiae TEF2Available sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
Plasmid#58441PurposeCassette to replace yeast promoters with an inducible promoter containing 3 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
UseTagsExpressionYeastMutationPromoterPTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR L1-vas2-Cre-L4
Plasmid#62301PurposeCre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoterDepositorInsertCre recombinase under control of vasa promoter and terminator
UseTagsHA and SV40 NLSExpressionInsectMutationPromotervasaAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTopo_Ex2Donor_pPGK-PURO
Plasmid#198862PurposeDonor template for CRISPR/Cas9 targeting of TERT Ex2DepositorInsertPURO
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioC
Plasmid#65349Purposesysthesis of vioC gene in the violacein pathwayDepositorInsertvioC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-sCS4-2T
Plasmid#28250DepositorInsertsingle chain Cse4-Scm3-H4 with 2 Thrombin site
UseTagsHisExpressionBacterialMutationA thrombin cutting site between Cse4 and Scm3. An…PromoterAvailable sinceJuly 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pME-nlsGal80(opt)
Plasmid#51618Purposevertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stopDepositorInsertGal80(opt) (GAL80 Budding Yeast)
UseVertebrate expressionTagsNLSExpressionMutationcodon optimizedPromoterAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUM31dao
Plasmid#87242PurposePlastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plantsDepositorInsertD-amino oxidase
UseTagsExpressionPlantMutationPromoterplastid rrnAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioE
Plasmid#65351Purposesysthesis of vioE gene in the violacein pathwayDepositorInsertvioE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-AktAR
Plasmid#123356PurposeFRET-based biosensor for monitoring Akt activity in lipid raft plasma membrane regions.DepositorInsertLyn-AktAR
UseTagsCerulean, N-terminal targeting sequence from Lyn …ExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ4
Plasmid#83559PurposeUsed to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.DepositorInsertGAL7 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL7Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUltra-5HTP
Plasmid#178042PurposeExpress modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codonDepositorInsertsModified ScTrpRS for 5HTP incoporation
suppressor tRNA for 5HTP incorporation
UseTagsExpressionBacterialMutationT107C, P254T, C255APromoterAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioA
Plasmid#65347Purposesysthesis of vioA gene in the violacein pathwayDepositorInsertvioA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMH-macroHC
Plasmid#82337PurposeProvides a C-terminal macro domain and 6xHis tag for expression in E. coliDepositorInsertSPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
UseTags6xHis non-cleavable and macro domain of human his…ExpressionBacterialMutationTruncated protein, amino acids 1-178PromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA
Plasmid#188704PurposeLentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2DepositorInsertNFATC2 sgRNAs
UseLentiviralTagsExpressionMammalianMutationPromoterhUbCAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
iRFP713-FRB-Mss4-Kina
Plasmid#202744Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorInsertiRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dom34(full length)
Plasmid#19098DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRB-HA no-pA
Plasmid#82594Purpose3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerizationDepositorInsertFRB-HA (no pA)
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSN074
Plasmid#166731PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS
UseCRISPRTagsSV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only