We narrowed to 59,355 results for: cloning

1. 1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

   Plasmid

   #171795

   Purpose

   pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

   Depositor

   Jean Giacomotto

   Article

   Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-NUDT5

   Plasmid

   #219954

   Purpose

   For gateway cloning of NUDT5

   Depositor

   Karla Feijs

   Article

   Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

   Insert

   NUDT5 (NUDT5 Human)
   

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR_hEMSY

   Plasmid

   #125156

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

   Insert

   EMSY (EMSY Human)
   

   Use

   Gateway entry vector

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRE:shRNA A-pGK:rtTA-Cre

   Plasmid

   #68471

   Purpose

   Inducible lentivirus expressing an arbitrary shRNA and Cre

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   GFP-shRNA A-miR30
   rtTA-2A-Cre
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   pGK and pTRE

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJOG824

   Plasmid

   #105401

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   SV40 nuclear localization signal
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   March 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZA8

   Plasmid

   #158486

   Purpose

   Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1
   

   Use

   Golden gate entry vector

   Mutation

   L17E, D481V, STOP codon

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO DNAJB4

   Plasmid

   #19472

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB4 (DNAJB4 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN_TT 3xFLAG Luciferase

   Plasmid

   #98391

   Purpose

   Gateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   Luciferase
   

   Use

   Entry vector for gateway cloning

   Tags

   3xFLAG

   Promoter

   TRE tight and TRE tight promoter

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJOG641

   Plasmid

   #105427

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   GAL4-VP16-GR (transcription factor: inducible expression; dexamethasone)
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-PT2A-GFP-iRFP670-Tdtomato

    Plasmid

    #111813

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    GFP
    iRFP670
    Tdtomato
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-PT2A-iRFP670-GFP-Tdtomato

    Plasmid

    #111816

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    iRFP670
    GFP
    Tdtomato
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQTEV-PEPD

    Plasmid

    #31328

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    peptidase D (PEPD Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTK_BsmBI_LacZ_Citrine_Nanotag_4

    Plasmid

    #130514

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    LacZ
    

    Use

    Enhancer cloning nanotag reporter vector

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221-kcnk3a

    Plasmid

    #208985

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk3a (kcnk3a Zebrafish)
    

    Use

    Cloning

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTK_BsmBI_LacZ_Cherry_Nanotag_105

    Plasmid

    #130546

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    LacZ
    

    Use

    Enhancer cloning nanotag reporter vector

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTEI042

    Plasmid

    #105351

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    Gal4UAS & 35S min promoter
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. S9_attL1-EGFP-C-term-attL2

    Plasmid

    #128473

    Purpose

    EGFP entry clone

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    EGFP entry clone
    

    Expression

    Bacterial

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-D-TOPO-kcnn3

    Plasmid

    #164959

    Purpose

    Zebrafish kcnn3 gene CDS

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

    Insert

    kcnn3 (kcnn3 Zebrafish)
    

    Use

    Pcr cloning vector

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS SK mCherryROSAbsr

    Plasmid

    #54321

    Purpose

    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    mCherry and BlasticidinS-resistance
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ-NF-L1-dMb2Cas12a

    Plasmid

    #177683

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dMb2Cas12a
    

    Use

    CRISPR; Gateway compatible foki-dmb2cas12a entry …

    Tags

    NLS

    Expression

    Plant

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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